Key features and details
- Goat polyclonal to Centaurin alpha 1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Product nameAnti-Centaurin alpha 1 antibody
See all Centaurin alpha 1 primary antibodies
DescriptionGoat polyclonal to Centaurin alpha 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Pig
- Human and mouse brain lysates for Western blot.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab27476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
1 hour primary incubation is recommended for this product.
RelevanceThe centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.
Cellular localizationCytoplasmic, Nuclear and Plasma membrane
- ArfGAP with dual PH domains 1 antibody
- CENTA 1 antibody
- CENTA1 antibody
All lanes : Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Pig brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab27476 has been referenced in 3 publications.
- Van Duzer A et al. ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival. Life Sci Alliance 2:N/A (2019). PubMed: 31792062
- Szatmari EM et al. Centaurin-a1-Ras-Elk-1 signaling at mitochondria mediates ß-amyloid-induced synaptic dysfunction. J Neurosci 33:5367-74 (2013). WB ; Rat . PubMed: 23516302
- Moore CD et al. The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation. J Cell Sci 120:2683-93 (2007). WB, ICC/IF, IHC-P ; Human, Rat . PubMed: 17635995