Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14285] to Centaurin alpha 1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Human
Product nameAnti-Centaurin alpha 1 antibody [EPR14285]
See all Centaurin alpha 1 primary antibodies
DescriptionRabbit monoclonal [EPR14285] to Centaurin alpha 1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human Centaurin alpha 1 aa 150-250. The exact sequence is proprietary.
Database link: O75689
- human fetal brain tissue lysate, A431 cell line lysate, U87 MG cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab183038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/20000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).|
|IP||1/20 - 1/40.|
RelevanceThe centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.
Cellular localizationCytoplasmic, Nuclear and Plasma membrane
- ArfGAP with dual PH domains 1 antibody
- CENTA 1 antibody
- CENTA1 antibody
Immunoprecipitation analysis of Human fetal brain lysate labeling Centaurin alpha 1 using ab183038 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
Immunofluorescent analysis of 4% paraformaldehyde fixed U87 MG cells labeling Centaurin alpha 1 with ab183038 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 (red). The cells are also seen stained with dapi (blue).
Anti-Centaurin alpha 1 antibody [EPR14285] (ab183038) at 1/20000 dilution + Human fetal brain tissue lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Anti-Centaurin alpha 1 antibody [EPR14285] (ab183038) at 1/5000 dilution + A431 cell line lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 43 kDa
ab183038 has not yet been referenced specifically in any publications.