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Goat polyclonal to CENTB1
Predicted to work with:
Mouse, Rat, Cow, Dog
Synthetic peptide: EKLSRRSHDLHTL, corresponding to C terminal amino acids 728-740 of Human CENTB1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 0.05% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 80-90 kDa (predicted molecular weight: 85 kDa).Can be blocked with
Human CENTB1 peptide (ab23280).
GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface.
Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.
Contains 3 ANK repeats.
Contains 1 Arf-GAP domain. Contains 1 BAR domain. Contains 1 PH domain.
PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes.
Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
Information by UniProt
ACAP 1 antibody
Western blot - Anti-CENTB1 antibody (ab15903)
Anti-CENTB1 antibody (ab15903) at 1 µg/ml + Jurkat cell lysate at 35 µg
Predicted band size:
Additional bands at:
80 kDa. We are unsure as to the identity of these extra bands.
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"