Anti-CNTLN antibody (ab122356)
Key features and details
- Rabbit polyclonal to CNTLN
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CNTLN antibody -
Description
Rabbit polyclonal to CNTLN -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CNTLN aa 289-379.
Sequence:LIEARKEVEVSQSKYNALSLQLSNKQTELIQKDMDITLVRKELQELQNLY KQNSTHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESIS
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Positive control
- Human liver tissue; Human liver lysate; Human cell line U-251 MG
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. Colocalizes with gamma-tubulin during interphase and mitosis. Appears to associated with the mother centriole during G1 phase and with daughter centrioles towards G1/S phase. - Information by UniProt
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Database links
- Entrez Gene: 54875 Human
- Omim: 611870 Human
- SwissProt: Q9NXG0 Human
- Unigene: 435381 Human
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Alternative names
- bA340N12.1 antibody
- C9orf101 antibody
- C9orf39 antibody
see all
Images
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ab122356, at 1/60 dilution, staining CNTLN in paraffin-embedded Human liver tissue by Immunohistochemistry.
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All lanes : Anti-CNTLN antibody (ab122356) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate -
ab122356, at 4 µg/ml, staining CNTLN in Human cell line U-251 MG by Immunofluorescence. Antibody staining is shown in green.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122356 has not yet been referenced specifically in any publications.