Overview

  • Product name

  • Description

    Rabbit polyclonal to Centrin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1-162 of Human Centrin 1 (AAH29515).

  • Positive control

    • Jurkat whole cell lysate (ab7899)

Properties

Applications

Our Abpromise guarantee covers the use of ab101332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • Immunocytochemical analysis of HeLa cells labelling Centrin 1 with ab101332. Counterstained with DAPI and computationally merged.

    See Abreview

  • Anti-Centrin 1 antibody (ab101332) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 20 kDa



    12% SDS PAGE

References

This product has been referenced in:

  • Muroyama A & Lechler T A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis. Elife 6:N/A (2017). Read more (PubMed: 28869035) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% TritonX100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 11 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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