Product nameAnti-Centrin 2 with anti-GAPDH internal loading control antibody
DescriptionRabbit polyclonal to Centrin 2 with anti-GAPDH internal loading control
Tested applicationsSuitable for: WBmore details
Unsuitable for: ChIP,Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human Centrin 2 with anti-GAPDH internal loading control aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This cocktail gave a positive signal in Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab80863 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 19, 37 kDa (predicted molecular weight: 19, 37 kDa). ab80863 contains 2 antibodies, generated by raising against a Centrin 2 immunogen and apiking with anti-GAPDH loading control.|
FunctionPlays a fundamental role in microtubule-organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CEP110.
Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer.
The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair.
Sequence similaritiesBelongs to the centrin family.
Contains 4 EF-hand domains.
Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Nucleus. Centrosome of S-phase, interphase and mitotic cells.
- Information by UniProt
- CALT antibody
- Caltractin (20kD calcium-binding protein) antibody
- Caltractin isoform 1 antibody
Anti-Centrin 2 with anti-GAPDH internal loading control antibody (ab80863) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19, 37 kDa
Observed band size: 19,37 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
The 19 and 37 kDa bands correlate to Centrin 2 and GAPDH, respectively.
ab80863 has not yet been referenced specifically in any publications.