Overview

  • Product name

  • Description

    Rabbit polyclonal to Centrin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Centrin 3 (internal sequence). The exact sequence is proprietary.
    Database link: O15182

  • Positive control

    • ICC/IF: A549 cells. WB: A549, NCI-H1299, HCT 116, C8D30, Neuro-2a, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts. IHC-P: Mouse cervix tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-Centrin 3 antibody (ab228690) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
    Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
    Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 20 kDa



    15% SDS-PAGE gel.

  • Paraffin-embedded mouse cervix tissue stained for Centrin 3 using ab228690 at 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-Centrin 3 antibody (ab228690) at 1/1000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 7 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 20 kDa



    15% SDS-PAGE gel.

  • Methanol-fixed A549 (human lung carcinoma cell line) cells stained for Centrin 3 (green) using ab228690 at 1/1000 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue). alpha Tubulin was stained with an anti-alpha Tubulin antibody (red) at 1/500 dilution.

References

ab228690 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228690.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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