Anti-CEP128 antibody (ab118797)
Key features and details
- Rabbit polyclonal to CEP128
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CEP128 antibody
See all CEP128 primary antibodies -
Description
Rabbit polyclonal to CEP128 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within amino acids 1044-1094 of Human CEP128 (NP_689659.2).
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Positive control
- 293T and HeLa whole cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 128 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 128 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Associates with the mother centriole. - Information by UniProt
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Database links
- Entrez Gene: 145508 Human
- SwissProt: Q6ZU80 Human
- Unigene: 162889 Human
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Alternative names
- C14orf145 antibody
- C14orf61 antibody
- CE128_HUMAN antibody
see all
Images
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All lanes : Anti-CEP128 antibody (ab118797) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 128 kDa
Exposure time: 3 minutes -
Detection of CEP128 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118797 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab118797 has been referenced in 2 publications.
- Chong WM et al. Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages. Elife 9:N/A (2020). PubMed: 32242819
- Viol L et al. Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis. J Cell Biol 219:N/A (2020). PubMed: 32211891