Overview

  • Product name

  • Description

    Rabbit polyclonal to CEP135
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CEP135 aa 900-950. The exact sequence is proprietary. NP_079285.2
    Database link: Q66GS9

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab245647 was affinity purified using an epitope specific to CEP135 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab245647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 5-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
    • Involvement in disease

      Microcephaly 8, primary, autosomal recessive
    • Sequence similarities

      Belongs to the CEP135/TSGA10 family.
    • Cellular localization

      Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
    • Information by UniProt
    • Database links

    • Alternative names

      • centrosomal protein 135 kDa antibody
      • centrosomal protein 135kDa antibody
      • Centrosomal protein 4 antibody
      • Centrosomal protein of 135 kDa antibody
      • centrosome protein 4 antibody
      • centrosome protein cep135 antibody
      • Cep135 antibody
      • CEP4 antibody
      • CP135_HUMAN antibody
      see all

    Images

    • CEP135 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

      ab245647 used for IP at 10 µg/mg lysate. For WB another anti-CEP135 was used at 1 µg/ml.

      Lane 1: ab245647 IP in HeLa whole cell lysate.
      Lane 2: Control IgG in HeLa whole cell lysate.

      Chemiluminescence detection: 30 seconds.

    References

    ab245647 has not yet been referenced specifically in any publications.

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