Key features and details
- Rabbit polyclonal to CEP135
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CEP135 antibody
See all CEP135 primary antibodies
DescriptionRabbit polyclonal to CEP135
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human CEP135 aa 900-950. The exact sequence is proprietary. NP_079285.2
Database link: Q66GS9
- IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245647 was affinity purified using an epitope specific to CEP135 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 5-10 µg/mg of lysate.|
FunctionCentrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
Involvement in diseaseMicrocephaly 8, primary, autosomal recessive
Sequence similaritiesBelongs to the CEP135/TSGA10 family.
Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
- Information by UniProt
- centrosomal protein 135 kDa antibody
- centrosomal protein 135kDa antibody
- Centrosomal protein 4 antibody
CEP135 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245647 used for IP at 10 µg/mg lysate. For WB another anti-CEP135 was used at 1 µg/ml.
Lane 1: ab245647 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245647 has not yet been referenced specifically in any publications.