Overview

  • Product name

  • Description

    Rabbit polyclonal to CEP135
  • Host species

    Rabbit
  • Specificity

    ab75005 detects endogenous levels of total CEP135 protein.
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    synthesized peptide derived from C-terminal region of human CEP135.

  • Positive control

    • extracts from COLO cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab75005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/500 - 1/1000. Predicted molecular weight: 134 kDa.
ELISA 1/40000.

Target

  • Function

    Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
  • Involvement in disease

    Microcephaly 8, primary, autosomal recessive
  • Sequence similarities

    Belongs to the CEP135/TSGA10 family.
  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
  • Information by UniProt
  • Database links

  • Alternative names

    • centrosomal protein 135 kDa antibody
    • centrosomal protein 135kDa antibody
    • Centrosomal protein 4 antibody
    • Centrosomal protein of 135 kDa antibody
    • centrosome protein 4 antibody
    • centrosome protein cep135 antibody
    • Cep135 antibody
    • CEP4 antibody
    • CP135_HUMAN antibody
    see all

Images

  • All lanes : Anti-CEP135 antibody (ab75005) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 134 kDa
    Observed band size: 134 kDa

References

This product has been referenced in:

  • Flanagan AM  et al. Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification. Mol Biol Cell 28:736-745 (2017). Read more (PubMed: 28100636) »
  • Sgourdou P  et al. Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly. Sci Rep 7:43708 (2017). Read more (PubMed: 28272472) »
See all 16 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thanks for your reply.
It does seem that the vial of ab75005 you received is not working as expected. I am very sorry for the trouble. If you have received this antibody within the past 6 months or so I would be happy to offer a free replacement of any primary antibody, credit or refund.
In your reply, please include the PO number or Abcam order reference number associated with this purchase so I can process your request.

Read More

Answer

Thank you for your enquiry. I am sorry to hear this vial of ab75005 is giving you trouble.
To ensure that the cell lysates are not degraded, I was wondering if you had successfully blotted these same cell lysates with any other antibodies?
Thanks in advance for the additional information!

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jul 29 2010

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