Key features and details
- Rabbit polyclonal to CEP135
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CEP135 antibody
See all CEP135 primary antibodies
DescriptionRabbit polyclonal to CEP135
Specificityab75005 detects endogenous levels of total CEP135 protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
synthesized peptide derived from C-terminal region of human CEP135.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab75005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 134 kDa.|
FunctionCentrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
Involvement in diseaseMicrocephaly 8, primary, autosomal recessive
Sequence similaritiesBelongs to the CEP135/TSGA10 family.
Cellular localizationCytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
- Information by UniProt
- centrosomal protein 135 kDa antibody
- centrosomal protein 135kDa antibody
- Centrosomal protein 4 antibody
ab75005 has been referenced in 18 publications.
- Khouj EM et al. Differential requirements for the EF-hand domains of human centrin 2 in primary ciliogenesis and nucleotide excision repair. J Cell Sci 132:N/A (2019). PubMed: 31492759
- Yaguchi K et al. Tetraploidy-associated centrosome overduplication in mouse early embryos. Commun Integr Biol 11:e1526605 (2018). PubMed: 30534347
- Flanagan AM et al. Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification. Mol Biol Cell 28:736-745 (2017). PubMed: 28100636
- Sgourdou P et al. Disruptions in asymmetric centrosome inheritance and WDR62-Aurora kinase B interactions in primary microcephaly. Sci Rep 7:43708 (2017). PubMed: 28272472
- Watanabe Y et al. Autophagy controls centrosome number by degrading Cep63. Nat Commun 7:13508 (2016). PubMed: 27869116