Overview

  • Product name

    Anti-CEP135 antibody - C-terminal
    See all CEP135 primary antibodies
  • Description

    Rabbit polyclonal to CEP135 - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab196809 detects endogenous levels of total CEP135 protein.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human CEP135 (C terminal). The exact sequence is proprietary.
    Database link: Q66GS9

  • Positive control

    • COLO cell extracts

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 134 kDa.

Target

  • Function

    Centrosomal protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole.
  • Involvement in disease

    Microcephaly 8, primary, autosomal recessive
  • Sequence similarities

    Belongs to the CEP135/TSGA10 family.
  • Cellular localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. During centriole biogenesis, it is concentrated within the proximal lumen of both parental centrioles and procentrioles.
  • Information by UniProt
  • Database links

  • Alternative names

    • centrosomal protein 135 kDa antibody
    • centrosomal protein 135kDa antibody
    • Centrosomal protein 4 antibody
    • Centrosomal protein of 135 kDa antibody
    • centrosome protein 4 antibody
    • centrosome protein cep135 antibody
    • Cep135 antibody
    • CEP4 antibody
    • CP135_HUMAN antibody
    see all

Images

  • All lanes : Anti-CEP135 antibody - C-terminal (ab196809) at 1/500 dilution

    Lane 1 : COLO cell extract
    Lane 2 : COLO cell extract with synthesized peptide

    Predicted band size: 134 kDa

References

This product has been referenced in:

  • Wang M  et al. A basal cell defect promotes budding of prostatic intraepithelial neoplasia. J Cell Sci 130:104-110 (2017). Read more (PubMed: 27609833) »
  • Tsuchiya Y  et al. Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole. Nat Commun 7:12567 (2016). Read more (PubMed: 27562453) »
See all 2 Publications for this product

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