Key features and details
- Rabbit polyclonal to CEP152
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CEP152 antibody
See all CEP152 primary antibodies
DescriptionRabbit polyclonal to CEP152
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human CEP152 aa 58-264. The exact sequence is proprietary.
Database link: O94986
- WB: HEK-293T and HeLa whole cell lysate (ab150035). ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab183911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 189 kDa.|
|ICC/IF||1/100 - 1/1000.|
Involvement in diseaseDefects in CEP152 are the cause of microcephaly primary type 4 (MCPH4) [MIM:604321]. A disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Affected individuals are mentally retarded. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits due to degenerative brain disorder.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Asterless antibody
- CE152_HUMAN antibody
- Centrosomal protein 152kDa antibody
Immunofluorescent analysis of HeLa cells (fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min) labeling CEP152 with ab183911 at 1/2000 dilution. Right panel costained with Hoechst 33342 (blue) and alpha 1b tubulin antibody at 1/5000 dilution (red).
All lanes : Anti-CEP152 antibody (ab183911) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 189 kDa
ab183911 has been referenced in 7 publications.
- Kim J et al. PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis. J Cell Sci 132:N/A (2019). PubMed: 30814333
- Fang CT et al. HSP70 is required for the proper assembly of pericentriolar material and function of mitotic centrosomes. Cell Div 14:4 (2019). PubMed: 31110557
- So C et al. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science 364:N/A (2019). PubMed: 31249032
- Yaguchi K et al. Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals. J Cell Biol 217:2463-2483 (2018). PubMed: 29712735
- Lee IW et al. Distinct roles of Cep192 and Cep152 in acentriolar MTOCs and spindle formation during mouse oocyte maturation. FASEB J 32:625-638 (2018). PubMed: 28970258
- Seo MY & Rhee K Caspase-mediated cleavage of the centrosomal proteins during apoptosis. Cell Death Dis 9:571 (2018). PubMed: 29752437
- Karki M et al. Precocious centriole disengagement and centrosome fragmentation induced by mitotic delay. Nat Commun 8:15803 (2017). ICC/IF ; Human . PubMed: 28607478