Product nameAnti-CEP170 antibody
See all CEP170 primary antibodies
DescriptionRabbit polyclonal to CEP170
Tested applicationsSuitable for: ICC/IF, ICC, WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow, Chimpanzee
Synthetic peptide corresponding to a region between residue 1534 and the C-teriminus (residue 1584) of human CEP170 (NP_055627.2)
- HeLa whole cell lysate (ab150035). This antibody gave a positive result in IHC in the following FFPE tissue: Human Testis.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72505 was affinity purified using an epitope specific to CEP170 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20890278|
|ICC||1/100 - 1/500.
Formaldehyde fixation with Triton-X 100 permeabilization is recommended.
|WB||1/2000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 176 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceCEP170, centrosomal protein 170kDa, plays a role in microtubule organization. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes.
Cellular localizationCentrosome, centriole. Cytoplasm, cytoskeleton. Associated with the mature mother centriole. Associated with spindle microtubules during mitosis.
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Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling CEP170 with ab72505 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
IHC image of CEP170 staining in Human Testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72505, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-CEP170 antibody (ab72505) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 176 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Detection of CEP170 by Western Blot of Immunprecipitate.
ab72505 at 1µg/ml staining CEP170 in HeLa whole cell lysate immunoprecipitated using ab72505 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
This product has been referenced in:
- Song K et al. A SEPT1-based scaffold is required for Golgi integrity and function. J Cell Sci 132:N/A (2019). Read more (PubMed: 30709970) »
- Lin JR et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). Read more (PubMed: 29993362) »