• Product name
  • Description
    Rabbit polyclonal to CEP170
  • Host species
  • Tested applications
    Suitable for: ICC/IF, ICC, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1534 and the C-teriminus (residue 1584) of human CEP170 (NP_055627.2)

  • Positive control
    • HeLa whole cell lysate (ab150035). This antibody gave a positive result in IHC in the following FFPE tissue: Human Testis.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab72505 was affinity purified using an epitope specific to CEP170 immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab72505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20890278
ICC 1/100 - 1/500.

Formaldehyde fixation with Triton-X 100 permeabilization is recommended.

WB 1/2000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 176 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Relevance
    CEP170, centrosomal protein 170kDa, plays a role in microtubule organization. During interphase, the encoded protein localizes to the sub-distal appendages of mature centrioles, which are microtubule-based structures thought to help organize centrosomes. During mitosis, the protein associates with spindle microtubules near the centrosomes.
  • Cellular localization
    Centrosome, centriole. Cytoplasm, cytoskeleton. Associated with the mature mother centriole. Associated with spindle microtubules during mitosis.
  • Database links
  • Alternative names
    • XRCC5 binding protein antibody
    • 4933426L22Rik antibody
    • A330004A13Rik antibody
    • AI195353 antibody
    • Centrosomal protein 170kDa antibody
    • Cep170 antibody
    • FAM68A antibody
    • KAB antibody
    • KARP 1 binding protein antibody
    • KARP1 binding protein antibody
    • KIAA0470 antibody
    see all


  • Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling CEP170 with ab72505 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • IHC image of CEP170 staining in Human Testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72505, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-CEP170 antibody (ab72505) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 176 kDa
    Observed band size: 220 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 30 seconds
  • Detection of CEP170 by Western Blot of Immunprecipitate.
    ab72505 at 1µg/ml staining CEP170 in HeLa whole cell lysate immunoprecipitated using ab72505 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.


This product has been referenced in:
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


Thank you very much for providing that information for me. It will be very helpful to us.

I have just had a look at the status of your order of ab43840 and it has been shipped today so will therefore be with you tomorrow as I said. Please do let me know how you get on with this antibody.

Until then, I wish you all the best with your research.

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Thank you for confirming those details.

I have now arranged for the new vial ofab43840 to be sent out to you. This is on the order number 1097435(Purchase order number FOCR 303778). This should get to you tomorrow. If you have any problems receiving it please dolet me know.

As mentioned in my previous email, it would be very useful for us to look into the problems you have had with ab72505. To this end, if you could share with me the protocol details and some images of the results I would be very grateful.

If you encounterany further problemswith the new antibody ab43840 please dolet me know. I look forward to receiving your reply.

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Thank you for contacting us yesterday and sorry for the delay in getting back to you.

I was sorry to hear that youhavebeenhavingdifficulties with the anti-CEP170 antibody(ab43840). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As discussed over the phone, I would be happy to arrange forthereplacement antibody you requested ()to be sent to you. Could you please just confirm for me that the delivery address that I have for you is correct:

Once I have this confirmation I will arrange for the antibody ab43840 to be sent out to you.

I understand the problems you have encountered with this antibody and the optimisation you have had to perform in trying to make it work as expected. If possible, so that I can investigate this case further with thelab, would you be able to give me a little more detail about the experiment carried out? If you wouldn't mind could you please fill out the questionnaire I have attached to this email, including any images you have if possible. This information will also allowme to investigate this case internally and initiate additional testing where necessary.

I look forward to receiving your reply.

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