Anti-CEP295 antibody (ab122490)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-CEP295 antibody -
Description
Rabbit polyclonal to CEP295 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CEP295 aa 1877-1978.
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Positive control
- ICC/IF: U-2 OS cells. WB: Human liver lysate. IHC-P: Human prostate tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/250 - 1/500. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 85459 Human
- SwissProt: Q9C0D2 Human
- Unigene: 458418 Human
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Alternative names
- hCG_2036584 antibody
- K1731_HUMAN antibody
- Kiaa1731 antibody
- Protein KIAA1731 antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CEP295 antibody (ab122490)Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labeling CEP295 with ab12249 shows positivity in the plasma membrane and cytoplasm.
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Western blot - Anti-CEP295 antibody (ab122490)All lanes : Anti-CEP295 antibody (ab122490) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP295 antibody (ab122490)ab122490 at 1/200 dilution staining CEP295 in paraffin-embedded human prostate tissue by Immunohistochemistry.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Kim J et al. PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis. J Cell Sci 132:N/A (2019). Read more (PubMed: 30814333) »
- Seo MY & Rhee K Caspase-mediated cleavage of the centrosomal proteins during apoptosis. Cell Death Dis 9:571 (2018). Read more (PubMed: 29752437) »
