Product nameAnti-CEP295 antibody
DescriptionRabbit polyclonal to CEP295
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CEP295 aa 1877-1978.
- ICC/IF: U-2 OS cells. WB: Human liver lysate. IHC-P: Human prostate tissue.
Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab122490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
- Information by UniProt
- hCG_2036584 antibody
- K1731_HUMAN antibody
- Kiaa1731 antibody
- Protein KIAA1731 antibody
Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labeling CEP295 with ab12249 shows positivity in the plasma membrane and cytoplasm.
All lanes : Anti-CEP295 antibody (ab122490) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
ab122490 at 1/200 dilution staining CEP295 in paraffin-embedded human prostate tissue by Immunohistochemistry.
This product has been referenced in:
- Kim J et al. PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis. J Cell Sci 132:N/A (2019). Read more (PubMed: 30814333) »
- Seo MY & Rhee K Caspase-mediated cleavage of the centrosomal proteins during apoptosis. Cell Death Dis 9:571 (2018). Read more (PubMed: 29752437) »