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    cep295-antibody-ab122490.pdf

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Tags & Cell Markers Subcellular Markers Organelles Centrosome
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Anti-CEP295 antibody (ab122490)

  • Datasheet
Submit a review Submit a question References (6)

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Immunocytochemistry/ Immunofluorescence - Anti-CEP295 antibody (ab122490)
  • Western blot - Anti-CEP295 antibody (ab122490)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP295 antibody (ab122490)

Key features and details

  • Rabbit polyclonal to CEP295
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-CEP295 antibody
  • Description

    Rabbit polyclonal to CEP295
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human CEP295 aa 1877-1978.

  • Positive control

    • ICC/IF: U-2 OS cells. WB: Human liver lysate. IHC-P: Human prostate tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab122490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/250 - 1/500.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
Notes
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/250 - 1/500.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Target

  • Target information above from: UniProt accession Q9C0D2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 85459 Human
    • SwissProt: Q9C0D2 Human
    • Unigene: 458418 Human
    • Alternative names

      • hCG_2036584 antibody
      • K1731_HUMAN antibody
      • Kiaa1731 antibody
      • Protein KIAA1731 antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CEP295 antibody (ab122490)
      Immunocytochemistry/ Immunofluorescence - Anti-CEP295 antibody (ab122490)

      Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labeling CEP295 with ab12249 shows positivity in the plasma membrane and cytoplasm.

    • Western blot - Anti-CEP295 antibody (ab122490)
      Western blot - Anti-CEP295 antibody (ab122490)
      All lanes : Anti-CEP295 antibody (ab122490) at 1/250 dilution

      Lane 1 : RT 4 cell lysate
      Lane 2 : U 251 MG cell lysate
      Lane 3 : Human Plasma lysate
      Lane 4 : Human Liver lysate
      Lane 5 : Human Tonsil lysate

      Developed using the ECL technique.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP295 antibody (ab122490)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP295 antibody (ab122490)

      ab122490 at 1/200 dilution staining CEP295 in paraffin-embedded human prostate tissue by Immunohistochemistry.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (6)

    Publishing research using ab122490? Please let us know so that we can cite the reference in this datasheet.

    ab122490 has been referenced in 6 publications.

    • Atorino ES  et al. CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion. Nat Commun 11:903 (2020). PubMed: 32060285
    • Kim J  et al. PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis. J Cell Sci 132:N/A (2019). PubMed: 30814333
    • Seo MY & Rhee K Caspase-mediated cleavage of the centrosomal proteins during apoptosis. Cell Death Dis 9:571 (2018). PubMed: 29752437
    • Fong CS  et al. PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion. Mol Biol Cell 29:2801-2808 (2018). PubMed: 30230954
    • Sydor AM  et al. PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN. Elife 7:N/A (2018). PubMed: 30168418
    • Fu J  et al. Conserved molecular interactions in centriole-to-centrosome conversion. Nat Cell Biol 18:87-99 (2016). PubMed: 26595382

    Customer reviews and Q&As

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