Anti-CEP295 antibody (ab122490)
Key features and details
- Rabbit polyclonal to CEP295
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CEP295 antibody -
Description
Rabbit polyclonal to CEP295 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to Human CEP295 aa 1877-1978.
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Positive control
- ICC/IF: U-2 OS cells. WB: Human liver lysate. IHC-P: Human prostate tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/250 - 1/500.
|
|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
| Notes |
|---|
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/250 - 1/500. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 85459 Human
- SwissProt: Q9C0D2 Human
- Unigene: 458418 Human
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Alternative names
- hCG_2036584 antibody
- K1731_HUMAN antibody
- Kiaa1731 antibody
- Protein KIAA1731 antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CEP295 antibody (ab122490)
Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labeling CEP295 with ab12249 shows positivity in the plasma membrane and cytoplasm.
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Western blot - Anti-CEP295 antibody (ab122490)All lanes : Anti-CEP295 antibody (ab122490) at 1/250 dilution
Lane 1 : RT 4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP295 antibody (ab122490)ab122490 at 1/200 dilution staining CEP295 in paraffin-embedded human prostate tissue by Immunohistochemistry.
Protocols
Datasheets and documents
References (6)
ab122490 has been referenced in 6 publications.
- Atorino ES et al. CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion. Nat Commun 11:903 (2020). PubMed: 32060285
- Kim J et al. PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis. J Cell Sci 132:N/A (2019). PubMed: 30814333
- Seo MY & Rhee K Caspase-mediated cleavage of the centrosomal proteins during apoptosis. Cell Death Dis 9:571 (2018). PubMed: 29752437
- Fong CS et al. PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion. Mol Biol Cell 29:2801-2808 (2018). PubMed: 30230954
- Sydor AM et al. PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN. Elife 7:N/A (2018). PubMed: 30168418
- Fu J et al. Conserved molecular interactions in centriole-to-centrosome conversion. Nat Cell Biol 18:87-99 (2016). PubMed: 26595382
