Anti-CEP350 antibody [CL3423] (ab219831)
Key features and details
- Mouse monoclonal [CL3423] to CEP350
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-CEP350 antibody [CL3423]
See all CEP350 primary antibodies -
Description
Mouse monoclonal [CL3423] to CEP350 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CEP350 aa 1400-1550.
Database link: Q5VT06 -
Epitope
Binds to an epitope located within the peptide sequence LPDFVKQLRTRT as determined by overlapping synthetic peptides. -
Positive control
- IHC-P: Human breast cancer, placenta and colon tissues. ICC/IF: U251, A431 and U2OS cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL3423 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
Use a concentration of 2 - 10 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Relevance
CAP350, centrosome associated protein 350, is required for anchoring microtubules to the centrosomes and for the integrity of the microtubule network. It recruits PPARA to discrete subcellular compartments and thereby modulates PPARA activity. -
Cellular localization
Centrosome. Spindle. Nucleus. Note=Associated with mitotic spindles. Nuclear, in discrete foci. Associated with intermediate filaments. -
Database links
- Entrez Gene: 9857 Human
-
Alternative names
- CAP 350 antibody
- Centrosomal protein 350kDa antibody
- Centrosome associated protein 350 antibody
see all
Images
-
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U251 cells labeling CEP350 with ab219831 at 4 μg/ml (green). Microtubule and nuclear probes are visualized in red and blue, respectively.
-
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U2OS cells labeling CEP350 with ab219831 at 4 μg/ml (green). Microtubule and nuclear probes are visualized in red and blue, respectively.
-
Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cells labeling CEP350 with ab219831 at 4 μg/ml (green). Microtubule and nuclear probes are visualized in red and blue, respectively.
-
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CEP350 with ab219831 at 1/50 dilution.
-
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling CEP350 with ab219831 at 1/50 dilution.
-
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CEP350 with ab219831 at 1/50 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab219831 has been referenced in 2 publications.
- Gonçalves AB et al. CEP78 functions downstream of CEP350 to control biogenesis of primary cilia by negatively regulating CP110 levels. Elife 10:N/A (2021). PubMed: 34259627
- Douanne T et al. CYLD Regulates Centriolar Satellites Proteostasis by Counteracting the E3 Ligase MIB1. Cell Rep 27:1657-1665.e4 (2019). PubMed: 31067453