RecombinantRabMAb

Recombinant Anti-CEP55 antibody [EPR11944(B)] (ab170414)

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Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
  • Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
  • Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
  • Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
  • Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
  • Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
  • Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
  • Anti-CEP55 antibody [EPR11944(B)] (ab170414)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11944(B)] to CEP55
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CEP55 antibody [EPR11944(B)]
    See all CEP55 primary antibodies

  • Description

    Rabbit monoclonal [EPR11944(B)] to CEP55

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CEP55. The exact sequence is proprietary.
    Database link: Q53EZ4

  • Positive control

    • SW480, HeLa, and HepG2 cell lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11944(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/400. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB 1/5000 - 1/20000. Predicted molecular weight: 55 kDa.

For unpurified use at 1/10000 - 1/50000.
ICC/IF 1/400.

For unpurified use at 1/250 - 1/500.
Flow Cyt 1/170.

For unpurified use at 1/500 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.

  • Tissue specificity

    Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.

  • Post-translational
    modifications

    There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.

  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > centrosome. Cleavage furrow. Midbody. Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • C10orf3 antibody
    • cancer/testis antigen 111 antibody
    • Centrosomal protein 55kDa antibody
    • Centrosomal protein of 55 kDa antibody
    • CEP 55 antibody
    • Cep55 antibody
    • CEP55_HUMAN antibody
    • CT111 antibody
    • FLJ10540 antibody
    • Up regulated in colon cancer 6 antibody
    • Up-regulated in colon cancer 6 antibody
    • URCC 6 antibody
    • URCC6 antibody
    see all

Images

  • Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/5000 dilution + SW480 Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 55 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    Overlay histogram showing HepG2 cells stained with ab170414 (red line) at 1/170 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    ab170414 staining CEP55 in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/400). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    ab170414 staining CEP55 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/400). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size: 55 kDa



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Flow Cytometry - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    Flow cytometric analysis of permeabilized CEP55 cells labeling CEP55 with ab170414, unpurified (red) or a rabbit IgG (negative) (green).

  • Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Western blot - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    All lanes : Anti-CEP55 antibody [EPR11944(B)] (ab170414) at 1/20000 dilution (unpurified)

    Lane 1 : SW480 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Immunocytochemistry/ Immunofluorescence - Anti-CEP55 antibody [EPR11944(B)] (ab170414)

    Immunofluorescence analysis of HeLa cells, labeling CEP55 using ab170414, unpurified.

  • Anti-CEP55 antibody [EPR11944(B)] (ab170414)
    Anti-CEP55 antibody [EPR11944(B)] (ab170414)

References (8)

Publishing research using ab170414? Please let us know so that we can cite the reference in this datasheet.

ab170414 has been referenced in 8 publications.

  • Sáez JJ  et al. The exocyst controls lysosome secretion and antigen extraction at the immune synapse of B cells. J Cell Biol 218:2247-2264 (2019). PubMed: 31197029
  • Luo J  et al. MiR-195-5p Inhibits Proliferation and Induces Apoptosis of Non-Small Cell Lung Cancer Cells by Targeting CEP55. Onco Targets Ther 12:11465-11474 (2019). PubMed: 31920335
  • Gao K  et al. iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation. Cell Death Dis 9:528 (2018). PubMed: 29743530
  • Qadir F  et al. Transcriptome reprogramming by cancer exosomes: identification of novel molecular targets in matrix and immune modulation. Mol Cancer 17:97 (2018). PubMed: 30008265
  • Yin Y  et al. MiR-144 suppresses proliferation, invasion, and migration of breast cancer cells through inhibiting CEP55. Cancer Biol Ther 19:306-315 (2018). PubMed: 29561704
  • Peng T  et al. Centrosomal protein 55 activates NF-?B signalling and promotes pancreatic cancer cells aggressiveness. Sci Rep 7:5925 (2017). IHC . PubMed: 28724890
  • Zhang W  et al. Upregulation of centrosomal protein 55 is associated with unfavorable prognosis and tumor invasion in epithelial ovarian carcinoma. Tumour Biol 37:6239-54 (2016). WB, IHC . PubMed: 26615423
  • Xu ZY  et al. Cep55 regulates spindle organization and cell cycle progression in meiotic oocyte. Sci Rep 5:16978 (2015). PubMed: 26582107

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