Recombinant
RabMAb

Recombinant Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free (ab240159)

Overview

  • Product name

    Anti-CEP55 antibody [EPR11944(B)] - BSA and Azide free
    See all CEP55 primary antibodies
  • Description

    Rabbit monoclonal [EPR11944(B)] to CEP55 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CEP55. The exact sequence is proprietary.
    Database link: Q53EZ4

  • General notes

    Ab240159 is the carrier-free version of ab170414. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240159 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11944(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.

Target

  • Function

    Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis.
  • Tissue specificity

    Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.
  • Post-translational
    modifications

    There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > centrosome. Cleavage furrow. Midbody. Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.
  • Information by UniProt
  • Database links

  • Alternative names

    • C10orf3 antibody
    • cancer/testis antigen 111 antibody
    • Centrosomal protein 55kDa antibody
    • Centrosomal protein of 55 kDa antibody
    • CEP 55 antibody
    • Cep55 antibody
    • CEP55_HUMAN antibody
    • CT111 antibody
    • FLJ10540 antibody
    • Up regulated in colon cancer 6 antibody
    • Up-regulated in colon cancer 6 antibody
    • URCC 6 antibody
    • URCC6 antibody
    see all

Images

  • Overlay histogram showing HepG2 cells stained with ab170414 (red line) at 1/170 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

  • ab170414 staining CEP55 in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde. Samples were incubated with primary antibody (1/400). An Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

  • ab170414 staining CEP55 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/400). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

  • Flow cytometric analysis of permeabilized CEP55 cells labeling CEP55 with ab170414, unpurified (red) or a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

  • Immunofluorescence analysis of HeLa cells, labeling CEP55 using ab170414, unpurified.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170414).

References

ab240159 has not yet been referenced specifically in any publications.

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