Key features and details
- Mouse polyclonal to CEP57
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CEP57 antibody
See all CEP57 primary antibodies
DescriptionMouse polyclonal to CEP57
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Chimpanzee, Rhesus monkey
- CEP57 transfected 293T cell lysate; HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.4
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab169301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionCentrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring-like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2.
Sequence similaritiesBelongs to the translokin family.
DomainThe C-terminal region mediates the interaction with microtubules and is able to nucleate and bundles microtubules in vitro.
The centrosome localization domain (CLD) region mediates the localization to centrosomes and homooligomerization.
Cellular localizationNucleus. Cytoplasm. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- Centrosomal protein 57kDa antibody
- Centrosomal protein of 57 kDa antibody
- Cep57 antibody
All lanes : Anti-CEP57 antibody (ab169301) at 1 µg/ml
Lane 1 : CEP57-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Immunofluorescent analysis of HeLa cells labeling CEP57 with ab169301 at 10 µg/ml.
ab169301 has been referenced in 1 publication.
- Watanabe K et al. The Cep57-pericentrin module organizes PCM expansion and centriole engagement. Nat Commun 10:931 (2019). PubMed: 30804344