Key features and details
- Rabbit polyclonal to CEP63
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CEP63 antibody
DescriptionRabbit polyclonal to CEP63
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human CEP63 aa 1-495.
Database link: Q96MT8
- WB: K562, HeLa, NIH/3T3 and Raji whole cell lysates. IHC-P: Human prostate cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 81 kDa.|
|IHC-P||1/20 - 1/200.|
RelevanceRequired for normal spindle assembly. Plays a role in DNA damage response: following DNA double strand breaks (DSBs), it is delocalized from centrosomes, leading to inactivate spindle assembly and delay mitotic progression.
- Centrosomal protein 63kDa antibody
- Centrosomal protein of 63 kDa antibody
- centrosome protein CEP63 antibody
All lanes : Anti-CEP63 antibody (ab235513) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 81 kDa
Paraffin-embedded human prostate cancer tissue stained for CEP63 using ab235513 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235513 has not yet been referenced specifically in any publications.