Key features and details
- Rabbit polyclonal to CEP70 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CEP70 antibody - N-terminal
See all CEP70 primary antibodies
DescriptionRabbit polyclonal to CEP70 - N-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Recombinant fragment within Human CEP70 (N terminal). The exact sequence is proprietary.
Database link: Q8NHQ1
- ICC/IF: NT2/D1 cells. WB: NT2/D1 whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 70 kDa.|
|ICC/IF||1/100 - 1/1000.|
Sequence similaritiesContains 1 TPR repeat.
Cellular localizationCytoplasm > cytoskeleton > centrosome.
- Information by UniProt
- BITE antibody
- Centrosomal protein of 70 kDa antibody
- Cep70 antibody
Ice-cold methanol-fixed NT2/D1 (human malignant pluripotent embryonic carcinoma cell line) cells stained for CEP70 (green) using ab227456 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Anti-CEP70 antibody - N-terminal (ab227456) at 1/1000 dilution + NT2/D1 (human malignant pluripotent embryonic carcinoma cell line) at 30 µg
Predicted band size: 70 kDa
7.5% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227456 has not yet been referenced specifically in any publications.