Key features and details
- Rabbit polyclonal to CEP72
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CEP72 antibody
See all CEP72 primary antibodies
DescriptionRabbit polyclonal to CEP72
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla
Synthetic peptide corresponding to a region between residues 150 and 200 of human CEP72 protein (NP_060610.2)
- HeLa and 293T whole cell lysates.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.764% Sodium citrate, 1.815% Tris, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab75254 was affinity purified using an epitope specific to CEP72 immobilized on solid support.
Our Abpromise guarantee covers the use of ab75254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in the recruitment of key centrosomal proteins to the centrosome. Provides centrosomal microtubule-nucleation activity on the gamma-tubulin ring complexes (gamma-TuRCs) and has critical roles in forming a focused bipolar spindle, which is needed for proper tension generation between sister chromatids. Required for localization of KIZ/PLK1S1, AKAP9 and gamma-tubulin ring complexes (gamma-TuRCs).
Sequence similaritiesBelongs to the CEP72 family.
Contains 3 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Localizes to the centrosome and centrosome-surrounding particles throughout the cell cycle. These particles disappear after microtubules are depolymerized using nocodazole, suggesting that CEP72-associating particles localize in a microtubule-dependent manner.
- Information by UniProt
- Centrosomal protein 72kDa antibody
- Centrosomal protein of 72 kDa antibody
- Cep72 antibody
All lanes : Anti-CEP72 antibody (ab75254) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 3 minutes
Detection of CEP72 by Western Blot of Immunprecipitate.
ab75254, at 1µg/ml, staining CEP72 in HeLa whole cell lysate immunoprecipitated using ab75254 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab75254 has not yet been referenced specifically in any publications.