Overview

  • Product name
    Anti-CEP76 antibody
  • Description
    Rabbit polyclonal to CEP76
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Rhesus monkey, Opossum, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 30 of Human CEP76 (NP_079175.2).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
IP Use at 10 µg/mg of lysate.

Target

  • Function
    Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication.
  • Sequence similarities
    Belongs to the CEP76 family.
  • Developmental stage
    Expressed at low level in G1 and is induced in S and G2 phase, during which point centrioles have already commenced duplication (at protein level).
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Does not localize along the ciliary axoneme.
  • Information by UniProt
  • Database links
  • Alternative names
    • C18orf9 antibody
    • Centrosomal protein 76kDa antibody
    • Centrosomal protein of 76 kDa antibody
    • CEP 76 antibody
    • Cep76 antibody
    • CEP76_HUMAN antibody
    • Chromosome 18 open reading frame 9 antibody
    • FLJ12542 antibody
    • HsT1705 antibody
    see all

Images

  • All lanes : Anti-CEP76 antibody (ab86613) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 76 kDa
    Observed band size: 69 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 115 kDa, 305 kDa. We are unsure as to the identity of these extra bands.



    Detection: Chemiluminescence with exposure time of 3 minutes
  • Western Blot detection of CEP76 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86613 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with exposure time of 30 seconds


    All lanes : Anti-CEP76 antibody (ab86613) at 1 µg/ml

    Lane 1 : immunoprecipitated CEP76
    Lane 2 : control IgG

References

ab86613 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab86613.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up