Anti-CEP76 antibody (ab86613)
Key features and details
- Rabbit polyclonal to CEP76
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CEP76 antibody -
Description
Rabbit polyclonal to CEP76 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Rhesus monkey, Opossum, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between residues 1 and 30 of Human CEP76 (NP_079175.2).
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Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
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IP |
Use at 10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 76 kDa. |
IP
Use at 10 µg/mg of lysate. |
Target
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Function
Centrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication. -
Sequence similarities
Belongs to the CEP76 family. -
Developmental stage
Expressed at low level in G1 and is induced in S and G2 phase, during which point centrioles have already commenced duplication (at protein level). -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Does not localize along the ciliary axoneme. - Information by UniProt
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Database links
- Entrez Gene: 79959 Human
- Entrez Gene: 225659 Mouse
- Entrez Gene: 291540 Rat
- SwissProt: Q8TAP6 Human
- SwissProt: Q0VEJ0 Mouse
- Unigene: 236940 Human
- Unigene: 111928 Mouse
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Alternative names
- C18orf9 antibody
- Centrosomal protein 76kDa antibody
- Centrosomal protein of 76 kDa antibody
see all
Images
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All lanes : Anti-CEP76 antibody (ab86613) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 76 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa, 305 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure time of 3 minutes -
Western Blot detection of CEP76 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86613 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 30 seconds
All lanes : Anti-CEP76 antibody (ab86613) at 1 µg/ml
Lane 1 : immunoprecipitated CEP76
Lane 2 : control IgG
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab86613 has not yet been referenced specifically in any publications.