Product nameAnti-CEP76 antibody
DescriptionRabbit polyclonal to CEP76
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Rhesus monkey, Opossum, Orangutan
Synthetic peptide corresponding to a region between residues 1 and 30 of Human CEP76 (NP_079175.2).
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 76 kDa.|
|IP||Use at 10 µg/mg of lysate.|
FunctionCentrosomal protein involved in regulation of centriole duplication. Required to limit centriole duplication to once per cell cycle by preventing centriole reduplication.
Sequence similaritiesBelongs to the CEP76 family.
Developmental stageExpressed at low level in G1 and is induced in S and G2 phase, during which point centrioles have already commenced duplication (at protein level).
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > centrosome > centriole. Does not localize along the ciliary axoneme.
- Information by UniProt
- C18orf9 antibody
- Centrosomal protein 76kDa antibody
- Centrosomal protein of 76 kDa antibody
All lanes : Anti-CEP76 antibody (ab86613) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 76 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa, 305 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure time of 3 minutes
Western Blot detection of CEP76 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab86613 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with exposure time of 30 seconds
All lanes : Anti-CEP76 antibody (ab86613) at 1 µg/ml
Lane 1 : immunoprecipitated CEP76
Lane 2 : control IgG
ab86613 has not yet been referenced specifically in any publications.