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    cep78-antibody-ab84867.pdf

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Tags & Cell Markers Subcellular Markers Organelles Centrosome
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Anti-CEP78 antibody (ab84867)

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Abpromise

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Immunocytochemistry/ Immunofluorescence - Anti-CEP78 antibody (ab84867)

    Key features and details

    • Rabbit polyclonal to CEP78
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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    Overview

    • Product name

      Anti-CEP78 antibody
      See all CEP78 primary antibodies
    • Description

      Rabbit polyclonal to CEP78
    • Host species

      Rabbit
    • Tested applications

      Suitable for: ICC/IFmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Chimpanzee, Gorilla, Orangutan
    • Immunogen

      Synthetic peptide, corresponding to a region between residue 550 and 600 of human CEP78 (CAI40237.1)

    • Positive control

      • HeLa Cells.
    • General notes

      Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.09% Sodium azide
      Constituents: 0.1% BSA, Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified using an epitope specific to CEP78 immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Centrosome

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab84867 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ICC/IF
  • Application notes
    ICC/IF: 1/100 - 1/500. Permeabilization with Triton X 100 is recommended.

    Acetone fixation is not recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      CEP78 belongs to the CEP78 family and contains 3 LRR (leucine rich) repeats.
    • Cellular localization

      Cytoplasmic; centrosome
    • Database links

      • Entrez Gene: 84131 Human
      • SwissProt: Q5JTW2 Human
      • Unigene: 374421 Human
      • Alternative names

        • 5730599I05Rik antibody
        • C9orf81 antibody
        • Centrosomal protein 78 antibody
        • Centrosomal protein 78kDa antibody
        • Chromosome 9 open reading frame 81 antibody
        • D030027P05 antibody
        • FLJ12643 antibody
        • FLJ52093 antibody
        • IP63 antibody
        • MGC135040 antibody
        • RP11-336N8.5 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-CEP78 antibody (ab84867)
        Immunocytochemistry/ Immunofluorescence - Anti-CEP78 antibody (ab84867)
        ab84867 at 1/250 dilution staining CEP78 in asynchronous HeLa cells by Immunocytochemistry, NBF-fixed. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 used at a dilution of 1/100.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab84867? Please let us know so that we can cite the reference in this datasheet.

      ab84867 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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