Anti-CEP78 antibody (ab86188)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CEP78 antibody
See all CEP78 primary antibodies -
Description
Rabbit polyclonal to CEP78 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residues 639 and 689 of human CEP78 (CAI40237.1)
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Positive control
- HeLa and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab86188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP |
Application notes
IP: Use at 2 - 5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
CEP78 belongs to the CEP78 family and contains 3 LRR (leucine rich) repeats. -
Cellular localization
Cytoplasmic; centrosome -
Database links
- Entrez Gene: 84131 Human
- SwissProt: Q5JTW2 Human
- Unigene: 374421 Human
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Alternative names
- 5730599I05Rik antibody
- C9orf81 antibody
- Centrosomal protein 78 antibody
see all
Images
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Western blot - Anti-CEP78 antibody (ab86188)All lanes : Anti-CEP78 antibody (ab86188) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-CEP78 antibody (ab86188)Detection of human CEP78 in Immunoprecipitates of HeLa whole cell lysate (1 mg klysate for IP, 20% of IP loaded) using ab86188 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
References
ab86188 has not yet been referenced specifically in any publications.
