Anti-CEP78 antibody (ab86188)
Key features and details
- Rabbit polyclonal to CEP78
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CEP78 antibody
See all CEP78 primary antibodies -
Description
Rabbit polyclonal to CEP78 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Orangutan
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Immunogen
Synthetic peptide corresponding to a region between residues 639 and 689 of human CEP78 (CAI40237.1)
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Positive control
- HeLa and 293T whole cell lysates
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab86188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP |
WB: 1/2000 - 1/10000. Predicted molecular weight: 76 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
CEP78 belongs to the CEP78 family and contains 3 LRR (leucine rich) repeats. -
Cellular localization
Cytoplasmic; centrosome -
Database links
- Entrez Gene: 84131 Human
- SwissProt: Q5JTW2 Human
- Unigene: 374421 Human
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Alternative names
- 5730599I05Rik antibody
- C9orf81 antibody
- Centrosomal protein 78 antibody
see all
Images
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Western blot - Anti-CEP78 antibody (ab86188)All lanes : Anti-CEP78 antibody (ab86188) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-CEP78 antibody (ab86188)Detection of human CEP78 in Immunoprecipitates of HeLa whole cell lysate (1 mg klysate for IP, 20% of IP loaded) using ab86188 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
References (0)
ab86188 has not yet been referenced specifically in any publications.
