Key features and details
- Goat polyclonal to Ceramide synthase 1/LAG1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ceramide synthase 1/LAG1 antibody
See all Ceramide synthase 1/LAG1 primary antibodies
DescriptionGoat polyclonal to Ceramide synthase 1/LAG1
Specificityab85696 is expected to recognize both reported isoforms (NP_067090.1 and NP_937850.1).
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
- Human brain (cerebellum, cerebral cortex and frontal cortex) lysates.
This product was previously labelled as Ceramide synthase 1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85696 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab85696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 38 kDa).
Calculated MW of 38.0 kDa according to NP_937850.1. An additional band of unknown identity was also consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide. 1 hour primary incubation is recommended for this product.
FunctionMay be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked stearoyl- (C18) ceramide) in a fumonisin B1-independent manner.
Sequence similaritiesContains 1 TLC (TRAM/LAG1/CLN8) domain.
Cellular localizationEndoplasmic reticulum membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Isoform 1 may recycle from the Golgi to the endoplasmic reticulum.
- Information by UniProt
- Ceramide synthase 1 antibody
- CerS1 antibody
- CERS1_HUMAN antibody
Anti-Ceramide synthase 1/LAG1 antibody (ab85696) at 1 µg/ml + Human Frontal cortex lysate (in RIPA buffer) at 35 µg
Predicted band size: 38 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. An additional band of unknown identity was consistently observed at 85 kDa. This band was successfully blocked by incubation with the immunising peptide.
ab85696 has not yet been referenced specifically in any publications.