Anti-DPF3 antibody (ab127703)
Key features and details
- Rabbit polyclonal to DPF3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DPF3 antibody
See all DPF3 primary antibodies -
Description
Rabbit polyclonal to DPF3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human DPF3 aa 15-166.
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Positive control
- IMR32 whole cell lysate.
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General notes
Keep as concentrated solution.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab127703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
Target
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Function
Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development. -
Sequence similarities
Belongs to the requiem/DPF family.
Contains 1 C2H2-type zinc finger.
Contains 2 PHD-type zinc fingers. -
Domain
The PHD-type zinc fingers mediate the binding to acetylated histones. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8110 Human
- Entrez Gene: 70127 Mouse
- Entrez Gene: 299186 Rat
- Omim: 601672 Human
- SwissProt: Q92784 Human
- SwissProt: P58269 Mouse
- Unigene: 162868 Human
- Unigene: 151308 Mouse
see all -
Alternative names
- 2810403B03Rik antibody
- BAF45C antibody
- BRG1-associated factor 45C antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab127703 has not yet been referenced specifically in any publications.