Overview

  • Product name
  • Description
    Rabbit polyclonal to CERK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 85-335 of Human CERK (Uniprot ID: Q8TCT0).

  • Positive control
    • Human breast carcinoma tissue; H1299, HeLa, HepG2 and Raji whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.
  • Tissue specificity
    High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung.
  • Sequence similarities
    Contains 1 DAGKc domain.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acylsphingosine kinase antibody
    • Ceramide kinase antibody
    • Cerk antibody
    • CERK1_HUMAN antibody
    • dA59H18.2 antibody
    • dA59H18.3 antibody
    • hCERK antibody
    • KIAA1646 antibody
    • Lipid kinase 4 antibody
    • Lipid kinase LK4 antibody
    • LK4 antibody
    see all

Images

  • Anti-CERK antibody (ab155061) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS-PAGE.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CERK with ab155061 at 1/500 dilution.

References

ab155061 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155061.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up