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Rabbit polyclonal to CERK
Predicted to work with:
Recombinant fragment, corresponding to a region within amino acids 85-335 of Human CERK (Uniprot ID: Q8TCT0).
Human breast carcinoma tissue; H1299, HeLa, HepG2 and Raji whole cell lysates.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
1/100 - 1/1000.
Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides.
High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung.
Contains 1 DAGKc domain.
Information by UniProt
Acylsphingosine kinase antibody
Ceramide kinase antibody
Western blot - Anti-CERK antibody (ab155061)
Anti-CERK antibody (ab155061) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 60 kDa 7.5% SDS-PAGE.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERK antibody (ab155061)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CERK with ab155061 at 1/500 dilution.
has not yet been referenced specifically in any publications.
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