Key features and details
- Rabbit polyclonal to CERKL
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CERKL antibody
See all CERKL primary antibodies
DescriptionRabbit polyclonal to CERKL
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human CERKL aa 1-558.
MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRG IFEIGRDSCDVVLSERALRWRPIQPERPAGDSKYDLLCKEEFIELKDIFS VKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDLINLSEDHCDI WFRQFKKILAGFPNRPKSLKILLNPQSHKKEATQVYYEKVEPLLKLAGIK TDVTIMEYEGHALSLLKECELQGFDGGHRKPLFAIHWSVQRLFTGMQTLE PSVVCVGGDGSASEVAHALLLRAQKNAGMETDRILTPVRAQLPLGLIPAG STNVLAHSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMF GFGGRTLALAEKYRWMSPNQRRDFAVVKALAKLKAEDCEISFLPFNSSDD VQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLAPNTRL NNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPR NNTGGYNPEEEEDETASENCFPWNVDGDLMEVASEVHIRLHPRLISLYGG SMEEMIPK
Database link: Q49MI3
- WB: Mouse lung tissue lysate. IHC-P: Human prostate cancer and pancreatic tissues. ICC/IF: MCF7 cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab222828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 20,25,43,48,53,58,60,63 kDa).|
FunctionHas no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
Tissue specificityIsoform 1 and isoform 2 are expressed in adult retina, liver and pancreas as well as in fetal brain, lung and kidney. Isoform 3 is expressed in adult retina as well as in fetal lung and liver. Isoform 4 is expressed in adult retina, lung and kidney as well as in fetal lung and liver. Moderately expressed in retina, kidney, lung, testis, trachea, and pancreas. Weakly expressed in brain, placenta and liver.
Involvement in diseaseDefects in CERKL are the cause of retinitis pigmentosa type 26 (RP26) [MIM:608380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP26 inheritance is autosomal recessive.
Sequence similaritiesContains 1 DAGKc domain.
Developmental stageExpressed in fetal lung, kidney and brain.
modificationsPhosphorylated on serine residues.
Cellular localizationCytoplasm. Nucleus > nucleolus. Enriched in nucleoli. May shuttle between nucleus and cytoplasm. Isoform 5 is not enriched in the nucleoli and Cytoplasm. Nucleus > nucleolus. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum.
- Information by UniProt
- Ceramide kinase like protein antibody
- Ceramide kinase-like protein antibody
- CERKL antibody
Anti-CERKL antibody (ab222828) at 1/1000 dilution + Mouse lung tissue lysate
Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 20,25,43,48,53,58,60,63 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Paraffin-embedded human prostate cancer tissue were stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreatic tissue stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.
MCF7 (human breast adenocarcinoma cell line) cells stained for CERKL (green) using ab222828 at 1/100 dilution in ICC/IF.
ab222828 has not yet been referenced specifically in any publications.