Anti-CERKL antibody (ab222828)

Submit a review Submit a question

Overview

  • Product name

    Anti-CERKL antibody
    See all CERKL primary antibodies

  • Description

    Rabbit polyclonal to CERKL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein corresponding to Human CERKL aa 1-558.
    Sequence:

    MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRG IFEIGRDSCDVVLSERALRWRPIQPERPAGDSKYDLLCKEEFIELKDIFS VKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDLINLSEDHCDI WFRQFKKILAGFPNRPKSLKILLNPQSHKKEATQVYYEKVEPLLKLAGIK TDVTIMEYEGHALSLLKECELQGFDGGHRKPLFAIHWSVQRLFTGMQTLE PSVVCVGGDGSASEVAHALLLRAQKNAGMETDRILTPVRAQLPLGLIPAG STNVLAHSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMF GFGGRTLALAEKYRWMSPNQRRDFAVVKALAKLKAEDCEISFLPFNSSDD VQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLAPNTRL NNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPR NNTGGYNPEEEEDETASENCFPWNVDGDLMEVASEVHIRLHPRLISLYGG SMEEMIPK


    Database link: Q49MI3

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Mouse lung tissue lysate. IHC-P: Human prostate cancer and pancreatic tissues. ICC/IF: MCF7 cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    >95%

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab222828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 20,25,43,48,53,58,60,63 kDa).

Target

  • Function

    Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.

  • Tissue specificity

    Isoform 1 and isoform 2 are expressed in adult retina, liver and pancreas as well as in fetal brain, lung and kidney. Isoform 3 is expressed in adult retina as well as in fetal lung and liver. Isoform 4 is expressed in adult retina, lung and kidney as well as in fetal lung and liver. Moderately expressed in retina, kidney, lung, testis, trachea, and pancreas. Weakly expressed in brain, placenta and liver.

  • Involvement in disease

    Defects in CERKL are the cause of retinitis pigmentosa type 26 (RP26) [MIM:608380]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP26 inheritance is autosomal recessive.

  • Sequence similarities

    Contains 1 DAGKc domain.

  • Developmental stage

    Expressed in fetal lung, kidney and brain.

  • Post-translational
    modifications

    Phosphorylated on serine residues.

  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Enriched in nucleoli. May shuttle between nucleus and cytoplasm. Isoform 5 is not enriched in the nucleoli and Cytoplasm. Nucleus > nucleolus. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ceramide kinase like protein antibody
      • Ceramide kinase-like protein antibody
      • CERKL antibody
      • CERKL_HUMAN antibody
      • Retinitis pigmentosa 26 (autosomal recessive) antibody
      • RP26 antibody
      see all

    Images

    • Western blot - Anti-CERKL antibody (ab222828)
      Western blot - Anti-CERKL antibody (ab222828)
      Anti-CERKL antibody (ab222828) at 1/1000 dilution + Mouse lung tissue lysate

      Secondary
      Goat Anti-Rabbit IgG at 1/10000 dilution

      Predicted band size: 20,25,43,48,53,58,60,63 kDa
      Observed band size: 63 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (ab222828)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (ab222828)

      Paraffin-embedded human prostate cancer tissue were stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (ab222828)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (ab222828)

      Paraffin-embedded human pancreatic tissue stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-CERKL antibody (ab222828)
      Immunocytochemistry/ Immunofluorescence - Anti-CERKL antibody (ab222828)

      MCF7 (human breast adenocarcinoma cell line) cells stained for CERKL (green) using ab222828 at 1/100 dilution in ICC/IF.

    References

    ab222828 has not yet been referenced specifically in any publications.

    Publishing research using ab222828? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab222828.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com