Key features and details
- Rabbit polyclonal to CerS2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CerS2 antibody
See all CerS2 primary antibodies
DescriptionRabbit polyclonal to CerS2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
Recombinant fragment within Human CerS2 (internal sequence). The exact sequence is proprietary.
Database link: Q96G23
- ICC/IF: HeLa cells. WB: HEK-293T and HeLa whole cell extracts.
This product was previously labelled as Ceramide synthase 2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227501 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 45 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionSuppresses the growth of cancer cells. May be involved in sphingolipid synthesis.
Tissue specificityExpressed in kidney, liver, brain, heart, placenta and lung.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
Contains 1 TLC (TRAM/LAG1/CLN8) domain.
Cellular localizationNucleus membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- Ceramide synthase 2 antibody
- CerS 2 antibody
- CerS2 antibody
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CerS2 (green) using ab227501 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
All lanes : Anti-CerS2 antibody (ab227501) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HeLa (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227501 has not yet been referenced specifically in any publications.