Overview

  • Product name

  • Description

    Rabbit polyclonal to CERT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human CERT aa 1-45 (N terminal).
    Sequence:

    MSDNQSWNSS GSEEDPETES GPPVERCGVL SKWTNYIHGW QDRWV


    Database link: Q9Y5P4

  • Positive control

    • A431, HeLa, HepG2 and H1299 whole cell lysates; methanol-fixed A431 cells.
  • General notes

    Previously labelled as COL4A3BP. 

Properties

Applications

Our Abpromise guarantee covers the use of ab151285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May mediate the intracellular trafficking of ceramide in a non-vesicular manner.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 PH domain.
    Contains 1 START domain.
  • Domain

    The START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
    The PH domain targets the Golgi apparatus.
    The FFAT motif is required for interaction with VAPA and VAPB.
  • Post-translational
    modifications

    Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
  • Cellular localization

    Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha 3 type IV collagen binding protein antibody
    • C43BP_HUMAN antibody
    • Ceramide transfer protein antibody
    • Ceramide transporter antibody
    • CERT antibody
    • Cert protein antibody
    • CERTL antibody
    • col4a3bp antibody
    • Collagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
    • Collagen type IV alpha 3 binding protein antibody
    • Collagen type IV alpha-3-binding protein antibody
    • FLJ20597 antibody
    • Goodpasture antigen binding protein antibody
    • Goodpasture antigen-binding protein antibody
    • GPBP antibody
    • hCERT antibody
    • Lipid transfer protein CERTL antibody
    • Procollagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
    • StAR related lipid transfer (START) domain containing 11 antibody
    • StAR related lipid transfer protein 11 antibody
    • StAR-related lipid transfer protein 11 antibody
    • StARD 11 antibody
    • StARD11 antibody
    • START domain containing 11 antibody
    • START domain containing protein 11 antibody
    • START domain-containing protein 11 antibody
    see all

Images

  • Anti-CERT antibody (ab151285) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed A431 cells labeling SA1 with ab151285 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

ab151285 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab151285.
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