Overview

  • Product name

  • Description

    Rabbit polyclonal to CERT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human CERT.
    Database link: NP_005704.1

  • Positive control

    • In Western Blot, this antibody gave a positive signal in HeLa and 293T whole cell lysates. This antibody gave a positive result when used in the following formaldehyde/methanol fixed cell lines: DU145.
  • General notes

    Previously labelled as COL4A3BP. 

Properties

Applications

Our Abpromise guarantee covers the use of ab72536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notes
    IP: Use at 1 - 4µg/mg of lysate.
    WB: 1/2500 - 1/10000. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May mediate the intracellular trafficking of ceramide in a non-vesicular manner.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Contains 1 PH domain.
      Contains 1 START domain.
    • Domain

      The START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
      The PH domain targets the Golgi apparatus.
      The FFAT motif is required for interaction with VAPA and VAPB.
    • Post-translational
      modifications

      Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
    • Cellular localization

      Cytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha 3 type IV collagen binding protein antibody
      • C43BP_HUMAN antibody
      • Ceramide transfer protein antibody
      • Ceramide transporter antibody
      • CERT antibody
      • Cert protein antibody
      • CERTL antibody
      • col4a3bp antibody
      • Collagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • Collagen type IV alpha 3 binding protein antibody
      • Collagen type IV alpha-3-binding protein antibody
      • FLJ20597 antibody
      • Goodpasture antigen binding protein antibody
      • Goodpasture antigen-binding protein antibody
      • GPBP antibody
      • hCERT antibody
      • Lipid transfer protein CERTL antibody
      • Procollagen type IV alpha 3 (Goodpasture antigen) binding protein antibody
      • StAR related lipid transfer (START) domain containing 11 antibody
      • StAR related lipid transfer protein 11 antibody
      • StAR-related lipid transfer protein 11 antibody
      • StARD 11 antibody
      • StARD11 antibody
      • START domain containing 11 antibody
      • START domain containing protein 11 antibody
      • START domain-containing protein 11 antibody
      see all

    Images

    • All lanes : Anti-CERT antibody (ab72536) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Predicted band size: 71 kDa
      Observed band size: 71 kDa
      Additional bands at: 100 kDa, 28 kDa, 60 kDa, 70 kDa (possible isoform). We are unsure as to the identity of these extra bands.

    • ICC/IF image of ab72536 stained DU145. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72536 at (5 µg/mL) overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti Rabbit  (96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Detection of Human CERT by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72536 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

    References

    This product has been referenced in:

    • Perreault L  et al. Intracellular localization of diacylglycerols and sphingolipids influences insulin sensitivity and mitochondrial function in human skeletal muscle. JCI Insight 3:N/A (2018). Read more (PubMed: 29415895) »
    • Yamaji T  et al. Role of Intracellular Lipid Logistics in the Preferential Usage of Very Long Chain-Ceramides in Glucosylceramide. Int J Mol Sci 17:N/A (2016). Human . Read more (PubMed: 27775668) »
    See all 4 Publications for this product

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