Key features and details
- Rabbit polyclonal to CERT - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CERT antibody - N-terminal
See all CERT primary antibodies
DescriptionRabbit polyclonal to CERT - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
Recombinant fragment within Human CERT aa 1-181 (N terminal). The exact sequence is proprietary.
Database link: Q9Y5P4
- A431 and HeLa cell lysates. Human prostate tissue.
Previously labelled as COL4A3BP.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1.79% Tris glycine
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab194769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 7.5 µg/ml.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 71 kDa.|
FunctionMay mediate the intracellular trafficking of ceramide in a non-vesicular manner.
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 PH domain.
Contains 1 START domain.
DomainThe START domain recognizes ceramide and mediates the intermembrane transfer of ceramide.
The PH domain targets the Golgi apparatus.
The FFAT motif is required for interaction with VAPA and VAPB.
modificationsPhosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity.
Cellular localizationCytoplasm. Golgi apparatus. Endoplasmic reticulum. Preferentially localized to the Golgi apparatus.
- Information by UniProt
- Alpha 3 type IV collagen binding protein antibody
- C43BP_HUMAN antibody
- Ceramide transfer protein antibody
All lanes : Anti-CERT antibody - N-terminal (ab194769) at 1/5000 dilution
Lane 1 : A431 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue, labeling CERT using ab194769 at 7.5 µg/ml.
ab194769 has not yet been referenced specifically in any publications.