Overview

  • Product name
    Anti-Ceruloplasmin antibody
    See all Ceruloplasmin primary antibodies
  • Description
    Rabbit polyclonal to Ceruloplasmin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide within Human Ceruloplasmin aa 350-450 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab111803)

  • Positive control
    • This antibody gave a positive signal in Human plasma total protein lysate as well as Human liver tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 148 kDa (predicted molecular weight: 122 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • Sequence similarities
    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CERU_HUMAN antibody
    • Ceruloplasmin antibody
    • CP 2 antibody
    • CP antibody
    • CP2 antibody
    • Ferroxidase antibody
    see all

Images

  • All lanes : Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 122 kDa
    Observed band size: 148 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 18 kDa, 75 kDa, 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute
  • ab110449 staining ceruloplasmin in astroglial cells from rat by ICC/IF (Immunocytochemistry/immunofluorescence).  Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in 0.1% PBS and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody for 4 hours at 24°C. An undiluted Alexa Fluor® 488-conjugated Donkey polyclonal was used as the secondary antibody.

     

    See Abreview

  • Adult rat was perfused with 4% PFA in 0.1M PBS. Fluorescent staining was performed in duodenum sections with anti-Ceruloplasmin (ab110449) primary and Alexa 488 conjugated secondary antibody. This antibody stained gut enterocytes. Nucleus stained with Hoechst at high power magnification.

    See Abreview

  • Anti-Ceruloplasmin antibody (ab110449) at 1 µg/ml + Native Human Ceruloplasmin protein (ab77930) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 122 kDa


    Exposure time: 30 seconds

References

This product has been referenced in:
  • Liu H  et al. Brain Ceruloplasmin Expression After Experimental Intracerebral Hemorrhage and Protection Against Iron-Induced Brain Injury. Transl Stroke Res 10:112-119 (2019). Read more (PubMed: 30315404) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your comment.

Before offering a testing discount for new species, our Scientific Team need to check the sequence homology to see if the cross-reactivity is expected or not.

If the homology is higher than 95%, then there is normally high chance for cross-reactivity. For this case, the homology is below 90%,so it is unlikely that ab110449 recognizes porcine Ceruloplasmin.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More
Application
Western blot
Sample
Pig Tissue lysate - whole (endometrial tissue lysate)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
30 µg
Specification
endometrial tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 26 2015

Answer

Thank you for the reply.

I have checked the sequence homology between human and pig (Sus scrofa) and it is only 87%. This means that the probability of this antibody to recognize pig (porcine) Ceruloplasmin is low. Please advise your customer to consult the following websites for further information:

http://www.ncbi.nlm.nih.gov/protein/388890649?report=genbank&log$=prottop&blast_rank=40&RID=6B5Y8MFB01R

http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

Gracias po contactarnos.

En principio no habría ningún problema para conjugar estos anticuerpos. Lo que si es necesario tener en cuenta es que el kit de conjugación sea compatible con los buffers y aditivos en los que conserva el anticuerpo.
Ignoro si disponéis de un kit de conjugación de anticuerpos, si os hiciera falta existen en nuestro catálogo los kits EasyLink para llevar a cabo la conjugación de anticuerpos con distintas enzimas o fluorocromos.
https://www.abcam.com/index.html?pageconfig=resource&rid=13148]
En el apartado correspondiente a las cuestiones técnicas del uso de estos kits encontrarás los buffers compatibles con los reactivos del kit, así como cuestiones relativas a la purificación y la concentración del anticuerpo que resultan útiles antes de usarlos.
https://www.abcam.com/index.html?pageconfig=resource&rid=13156
En el primero de los links indicado hay a su vez links directos a los kits de conjugación EasyLink tanto con HRP como con AP.
Espero que esta información te sea útil, de lo contrario, por favor no dudes en volver a contactarnos.

Read More
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Duodenum)
Specification
Duodenum

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 05 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Astro-glia)
Specification
Astro-glia
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 02 2011

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