Overview

  • Product name
    Anti-Ceruloplasmin antibody
    See all Ceruloplasmin primary antibodies
  • Description
    Rabbit polyclonal to Ceruloplasmin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 555-584 of Human Ceruloplasmin.

  • Positive control
    • Mouse lung tissue lysate; Human hepatocarcinoma tissue; HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab135649 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 122 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • Sequence similarities
    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CERU_HUMAN antibody
    • Ceruloplasmin antibody
    • CP 2 antibody
    • CP antibody
    • CP2 antibody
    • Ferroxidase antibody
    see all

Images

  • Anti-Ceruloplasmin antibody (ab135649) at 1/50 dilution + Mouse lung tissue lysate at 35 µg

    Predicted band size: 122 kDa

  • Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma labelling Ceruloplasmin with ab135649 at 1/50 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
  • Flow cytometric analysis of HepG2 cells labelling Ceruloplasmin with ab135649 at 1/10 dilution (green) compared to a negative control cell (blue). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver lysate)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
20 µg
Specification
Liver lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 20 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up