• Product name
    Anti-Ceruloplasmin antibody
    See all Ceruloplasmin primary antibodies
  • Description
    Rabbit polyclonal to Ceruloplasmin
  • Host species
  • Tested applications
    Suitable for: IHC-P, IHC-FoFr, IP, RIA, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Human ceruloplasmin purified from human plasma


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, PBS, pH 7.5
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab48614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


  • Function
    Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • Sequence similarities
    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CERU_HUMAN antibody
    • Ceruloplasmin antibody
    • CP 2 antibody
    • CP antibody
    • CP2 antibody
    • Ferroxidase antibody
    see all


  • Anti-Ceruloplasmin antibody (ab48614) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 122 kDa
    Observed band size: 122 + 148 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 34 kDa, 76 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 30 seconds

    Ceruloplasmin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted (148 kDa).
  • Ab48614 staining human tonsil. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer, EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • IHC-FoFr image of ceruloplasmin staining on mouse duodenal sections using ab48614. These sections were incubated in triton X (0.3% in 0.1% PBS) to permeabilise the cells and in 10% serum for 1h to block non-specific protein-protein interactions. The sections were then incubated with the antibody ab48614(1:200) overnight at +4°C. Specific protien binding was visualised using an Alexa 488 conjugated donkey secondary antibody (green staining). Alexa 568 conjugated secondary was used to visualise ab55027 binding to ferritin (red staining).

    See Abreview


This product has been referenced in:
  • Zanardi A  et al. Ceruloplasmin replacement therapy ameliorates neurological symptoms in a preclinical model of aceruloplasminemia. EMBO Mol Med 10:91-106 (2018). Read more (PubMed: 29183916) »
  • Pelucchi S  et al. Phenotypic heterogeneity in seven Italian cases of aceruloplasminemia. Parkinsonism Relat Disord 51:36-42 (2018). WB ; Human . Read more (PubMed: 29503155) »
See all 5 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (10% PAGE)
Fish Serum (plasma)
Blocking step
(agent) for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Jan 26 2015


Thank you for your clarification.

I can confirm the concentration of ab66219 (Batch: GR9812) of this ab is 1 mg/ml. The other antibody ab36204 (Lot: GR42382) is not affinity purified but whole antiserum so the IgG is not quantified. However, I can provide a range of the IgG: 10-15 mg/ml.

Isotype controls:

can be searched at this site: https://www.abcam.com/index.html?c=718

ab36204, ab48614, ab66219, ab102998 --> Rabbit IgG - ab37406, ab125938, ab128142

ab83508 --> Mouse IgG1 - ab91353, ab27479, ab81032

Positive control:

ab36204 - CRMP4 - It is mainly expressed in heart and skeletal muscle and also strongly expressed in fetal brain and spinal cord (human, mouse or rat),

ab48614 --Ceruloplasmin - It is expressed by the liver and secreted in plasma,

ab66219 -- Oprl1 - Brain (cortex),

ab83508 -- NG2 - It is detected only in malignant melanoma cells, SK-MEL-28 cells,

ab102998 --B3GNT5- only tested in WB - Widely expressed. It is highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum, MDA-MB435 cell line

If you need any further assistance in the future, please do not hesitate to contact me.

Read More


Thank you for your enquiry and your interest in our products.

This is to confirm that we can only provide the concentration for affinity purified antibodies. For whole serum or supernatant we do not quantify the IgG concentration. I have contacted the lab to get the requested information for you concerning ab66219 (Lot: GR9812).

Would you be so kind to clarify if you wish to look for isotype control or positive control for these primary antibodies listed below?

ab36204 --> Rabbit IgG
ab48614 --> Rabbit IgG
ab66219 --> Rabbit IgG
ab83508 --> Mouse IgG1
ab102998 --> Rabbit IgG

I look forward to hearing from you soon. As soon as I have further information on the concentration (ab66219), I will contact you again.

Read More


Gracias po contactarnos.

En principio no habría ningún problema para conjugar estos anticuerpos. Lo que si es necesario tener en cuenta es que el kit de conjugación sea compatible con los buffers y aditivos en los que conserva el anticuerpo.
Ignoro si disponéis de un kit de conjugación de anticuerpos, si os hiciera falta existen en nuestro catálogo los kits EasyLink para llevar a cabo la conjugación de anticuerpos con distintas enzimas o fluorocromos.
En el apartado correspondiente a las cuestiones técnicas del uso de estos kits encontrarás los buffers compatibles con los reactivos del kit, así como cuestiones relativas a la purificación y la concentración del anticuerpo que resultan útiles antes de usarlos.
En el primero de los links indicado hay a su vez links directos a los kits de conjugación EasyLink tanto con HRP como con AP.
Espero que esta información te sea útil, de lo contrario, por favor no dudes en volver a contactarnos.

Read More
Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Cell (Duodenum)
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 02 2011

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