Recombinant
RabMAb

Recombinant Anti-Ceruloplasmin antibody [EPSISR6-12] (ab131220)

Overview

  • Product name
    Anti-Ceruloplasmin antibody [EPSISR6-12]
    See all Ceruloplasmin primary antibodies
  • Description
    Rabbit monoclonal [EPSISR6-12] to Ceruloplasmin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human Ceruloplasmin aa 400-500. The exact sequence is proprietary.

  • Positive control
    • WB: Human plasma; Human fetal kidney lysate; Rat liver lysate.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPSISR6-12
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 132 kDa (predicted molecular weight: 122 kDa).
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
    • Tissue specificity
      Expressed by the liver and secreted in plasma.
    • Involvement in disease
      Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
      Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
    • Sequence similarities
      Belongs to the multicopper oxidase family.
      Contains 3 F5/8 type A domains.
      Contains 6 plastocyanin-like domains.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • CERU_HUMAN antibody
      • Ceruloplasmin antibody
      • CP 2 antibody
      • CP antibody
      • CP2 antibody
      • Ferroxidase antibody
      see all

    Images

    • All lanes : Anti-Ceruloplasmin antibody [EPSISR6-12] (ab131220) at 1/1000 dilution

      Lane 1 : Human plasma lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Rat liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 122 kDa

    References

    This product has been referenced in:
    • Katerji M  et al. Chemosensitivity of U251 Cells to the Co-treatment of D-Penicillamine and Copper: Possible Implications on Wilson Disease Patients. Front Mol Neurosci 10:10 (2017). Read more (PubMed: 28197071) »
    • Chen TD  et al. Identification of Ceruloplasmin as a Gene that Affects Susceptibility to Glomerulonephritis Through Macrophage Function. Genetics 206:1139-1151 (2017). Read more (PubMed: 28450461) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Answer

    I can confirm that ab157452 and ab131220 were produced from same monoclonal antibody production project [EPSISR6] using the same immunogen. However, they are different clones that were produced from this project.

    They are tested and covered by the guarantee for different applications.

    ab157452 clone SISR6-42
    Suitable for :ICC, WB
    Reacts with: human

    ab131220 SISR6-12
    Suitable for WB
    Reacts with: rat, human

    Our datasheet team currently adding more detailed clone number information to the datasheets.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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