Overview

  • Product name

  • Description

    Rabbit polyclonal to CES2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 51-469 of Human CES2.

  • Positive control

    • HepG2 whole cell lysate; HepG2 cells; H1299 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab137606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
IHC-P 1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methyumbelliferyl acetate, heroin and 6-monoacetylmorphine.
  • Tissue specificity

    Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.
  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • Carboxylesterase 2 (intestine, liver) antibody
    • Carboxylesterase 2 antibody
    • CE-2 antibody
    • CES2 antibody
    • CES2A1 antibody
    • Cocaine esterase antibody
    • EC 3.1.1.1 antibody
    • EST2_HUMAN antibody
    • hCE-2 antibody
    • ICE antibody
    • Iintestinal carboxylesterase liver carboxylesterase 2 antibody
    • Intestinal carboxylesterase; liver carboxylesterase-2 antibody
    • Methylumbelliferyl acetate deacetylase 2 antibody
    • PCE 2 antibody
    • PCE-2 antibody
    see all

Images

  • Anti-CES2 antibody (ab137606) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg/ml

    Predicted band size: 62 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of HepG2 cells fixed in iced-cold MeOH for 5 minutes, labelling CES2 with ab137606 at 1/500 dilution (Green).
    Blue: Hoechst 33342 staining.
  • Immunohistochemical analysis of paraffin-embedded H1299 xenograft labelling CES2 with ab137606 at 1/500 dilution.

References

This product has been referenced in:

  • Li J  et al. Sortilin 1 Modulates Hepatic Cholesterol Lipotoxicity in Mice via Functional Interaction with Liver Carboxylesterase 1. J Biol Chem 292:146-160 (2017). Read more (PubMed: 27881673) »
  • Fu J  et al. Biotransformation Capacity of Carboxylesterase in Skin and Keratinocytes for the Penta-Ethyl Ester Prodrug of DTPA. Drug Metab Dispos 44:1313-8 (2016). Read more (PubMed: 27130352) »
See all 2 Publications for this product

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