Recombinant
RabMAb

Recombinant Anti-CES2 antibody [EPR14856] (ab184957)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14856] to CES2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CES2 antibody [EPR14856]
    See all CES2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14856] to CES2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CES2 aa 50-150. The exact sequence is proprietary.
    Database link: O00748

  • Positive control

    • HepG2 cell lysate, Human fetal liver lysate, Human colon tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab184957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methyumbelliferyl acetate, heroin and 6-monoacetylmorphine.

  • Tissue specificity

    Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.

  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.

  • Post-translational
    modifications

    Glycosylated.

  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt

  • Database links

    • Alternative names

      • Carboxylesterase 2 (intestine, liver) antibody
      • Carboxylesterase 2 antibody
      • CE-2 antibody
      • CES2 antibody
      • CES2A1 antibody
      • Cocaine esterase antibody
      • EC 3.1.1.1 antibody
      • EST2_HUMAN antibody
      • hCE-2 antibody
      • ICE antibody
      • Iintestinal carboxylesterase liver carboxylesterase 2 antibody
      • Intestinal carboxylesterase; liver carboxylesterase-2 antibody
      • Methylumbelliferyl acetate deacetylase 2 antibody
      • PCE 2 antibody
      • PCE-2 antibody
      see all

    Images

    • Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
      Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
      Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution + HepG2 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 62 kDa

    • Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
      Western blot - Anti-CES2 antibody [EPR14856] (ab184957)
      Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution + Human fetal liver lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 62 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (ab184957)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (ab184957)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CES2 with ab184957 at 1/500 dilution.  Counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    References

    Publishing research using ab184957? Please let us know so that we can cite the reference in this datasheet.

    ab184957 has been referenced in 1 publication.

    • Yu J  et al. High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. Oncotarget 7:75279-75292 (2016). WB, IHC-P ; Human . PubMed: 27661117

    Customer reviews and Q&As

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    Western blot abreview for Anti-CES2 antibody [EPR14856]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    30 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 13 2015

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