Recombinant Anti-CES2 antibody [EPR14856] (ab184957)
![Western blot - Anti-CES2 antibody [EPR14856] (ab184957)](https://www.abcam.com/ps/products/184/ab184957/Images/ab184957-217665-anti-ces2-antibody-epr14856-western-blot.jpg "Western blot - Anti-CES2 antibody [EPR14856] (ab184957)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14856] to CES2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CES2 antibody [EPR14856]
See all CES2 primary antibodies -
Description
Rabbit monoclonal [EPR14856] to CES2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2 cell lysate, Human fetal liver lysate, Human colon tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14856 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methyumbelliferyl acetate, heroin and 6-monoacetylmorphine. -
Tissue specificity
Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
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Database links
- Entrez Gene: 8824 Human
- Omim: 605278 Human
- SwissProt: O00748 Human
- Unigene: 282975 Human
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Alternative names
- Carboxylesterase 2 (intestine, liver) antibody
- Carboxylesterase 2 antibody
- CE-2 antibody
see all
Images
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Western blot - Anti-CES2 antibody [EPR14856] (ab184957)Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDa -
![Western blot - Anti-CES2 antibody [EPR14856] (ab184957)](https://www.abcam.com/ps/products/184/ab184957/Images/ab184957-217664-anti-ces2-antibody-epr14856-western-blot.jpg)
Western blot - Anti-CES2 antibody [EPR14856] (ab184957)Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (ab184957)](https://www.abcam.com/ps/products/184/ab184957/Images/ab184957-217663-anti-ces2-antibody-epr14856-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (ab184957)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CES2 with ab184957 at 1/500 dilution. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-CES2 antibody [EPR14856] (ab184957)](https://www.abcam.com/ps/products/184/ab184957/Images/ab184957-4-benefits-of-recombinant-antibodies.png)
Anti-CES2 antibody [EPR14856] (ab184957)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab184957 has been referenced in 1 publication.
- Yu J et al. High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. Oncotarget 7:75279-75292 (2016). WB, IHC-P ; Human . PubMed: 27661117
