Overview

  • Product name

    Anti-CES2 antibody [EPR14856]
    See all CES2 primary antibodies
  • Description

    Rabbit monoclonal [EPR14856] to CES2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CES2 aa 50-150. The exact sequence is proprietary.
    Database link: O00748

  • Positive control

    • HepG2 cell lysate, Human fetal liver lysate, Human colon tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IHC-P 1/500.

Target

  • Function

    Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methyumbelliferyl acetate, heroin and 6-monoacetylmorphine.
  • Tissue specificity

    Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.
  • Sequence similarities

    Belongs to the type-B carboxylesterase/lipase family.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Endoplasmic reticulum lumen.
  • Information by UniProt
  • Database links

  • Alternative names

    • Carboxylesterase 2 (intestine, liver) antibody
    • Carboxylesterase 2 antibody
    • CE-2 antibody
    • CES2 antibody
    • CES2A1 antibody
    • Cocaine esterase antibody
    • EC 3.1.1.1 antibody
    • EST2_HUMAN antibody
    • hCE-2 antibody
    • ICE antibody
    • Iintestinal carboxylesterase liver carboxylesterase 2 antibody
    • Intestinal carboxylesterase; liver carboxylesterase-2 antibody
    • Methylumbelliferyl acetate deacetylase 2 antibody
    • PCE 2 antibody
    • PCE-2 antibody
    see all

Images

  • Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Anti-CES2 antibody [EPR14856] (ab184957) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CES2 with ab184957 at 1/500 dilution.  Counterstained with Hematoxylin.

References

This product has been referenced in:

  • Yu J  et al. High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. Oncotarget 7:75279-75292 (2016). WB, IHC-P ; Human . Read more (PubMed: 27661117) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 13 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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