Anti-CES3/TGH antibody (ab215045)

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Western blot - Anti-CES3/TGH antibody (ab215045)

    Key features and details

    • Sheep polyclonal to CES3/TGH
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Overview

    • Product name

      Anti-CES3/TGH antibody
      See all CES3/TGH primary antibodies

    • Description

      Sheep polyclonal to CES3/TGH

    • Host species

      Sheep

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat

    • Immunogen

      Recombinant fragment corresponding to Mouse CES3/TGH aa 19-561. NS0-derived
      Sequence:

      YPSSPPVVNTVKGKVLGKYVNLEGFTQPVAVFLGVPFAKPPLGSLRFAPP QPAEPWSFVKNTTSYPPMCSQDAVGGQVLSELFTNRKENIPLQFSEDCLY LNIYTPADLTKNSRLPVMVWIHGGGLVVGGASTYDGLALSAHENVVVVTI QYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIANFGGNPGSVTIF GESAGGFSVSVLVLSPLAKNLFHRAISESGVSLTAALITTDVKPIAGLVA TLSGCKTTTSAVMVHCLRQKTEDELLETSLKLNLFKLDLLGNPKESYPFL PTVIDGVVLPKAPEEILAEKSFSTVPYIVGINKQEFGWIIPTLMGYPLAE GKLDQKTANSLLWKSYPTLKISENMIPVVAEKYLGGTDDLTKKKDLFQDL MADVVFGVPSVIVSRSHRDAGASTYMYEFEYRPSFVSAMRPKAVIGDHGD EIFSVFGSPFLKDGASEEETNLSKMVMKFWANFARNGNPNGGGLPHWPEY DQKEGYLKIGASTQAAQRLKDKEVSFWAELRAKESAQRPSHRE


      Database link: Q8VCT4
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • Mouse lung tissue lysate.

    • General notes

       This product was previously labelled as CES3

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Lyophilized:Reconstitute the antibody with 100 uL sterile PBS and the final concentration is 500 ug/mL. Reconstituted antibody can be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      Constituent: 100% PBS
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      The IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS).

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab215045 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/1000 - 1/2000. Predicted molecular weight: 62 kDa.

    Target

    • Function

      Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows low catalytic efficiency for hydrolysis of CPT-11 (7-ethyl-10-[4-(1-piperidino)-1-piperidino]-carbonyloxycamptothecin), a prodrug for camptothecin used in cancer therapeutics.

    • Tissue specificity

      Expressed in liver, colon and small intestine.

    • Sequence similarities

      Belongs to the type-B carboxylesterase/lipase family.

    • Post-translational
      modifications

      N-glycosylated.

    • Cellular localization

      Endoplasmic reticulum lumen.
    • Information by UniProt

    • Database links

      • Alternative names

        • Carboxylesterase 3 antibody
        • CES 3 antibody
        • Ces3 antibody
        • ES3 antibody
        • EST3_HUMAN antibody
        • FLJ21736 antibody
        • Liver carboxylesterase 31 homolog antibody
        see all

      Images

      • Western blot - Anti-CES3/TGH antibody (ab215045)
        Western blot - Anti-CES3/TGH antibody (ab215045)
        Anti-CES3/TGH antibody (ab215045) at 1/1000 dilution + mouse lung tissue lysate

        Predicted band size: 62 kDa

      References (0)

      Publishing research using ab215045? Please let us know so that we can cite the reference in this datasheet.

      ab215045 has not yet been referenced specifically in any publications.

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