Anti-CETP antibody (ab19012)
- Datasheet
- References (5)
- Protocols
Overview
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Product nameAnti-CETP antibody
See all CETP primary antibodies -
DescriptionRabbit polyclonal to CETP
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Host speciesRabbit
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SpecificityThis antibody recognises CETB (70 kDa).
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Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Rat, Rabbit, Human
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Immunogen
Synthetic peptide corresponding to Human CETP (N terminal). Immunogen is around amino acid 153.
Database link: P11597
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Alternative Products
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Assay kits
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab19012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA | ||
ICC/IF | ||
WB |
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 70 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionInvolved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.
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Tissue specificityExpressed by the liver and secreted in plasma.
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Involvement in diseaseDefects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels. -
Sequence similaritiesBelongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
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Cellular localizationSecreted > extracellular space.
- Information by UniProt
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Database links
- Entrez Gene: 1071 Human
- Omim: 118470 Human
- SwissProt: P11597 Human
- SwissProt: P22687 Rabbit
- Unigene: 89538 Human
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Alternative names
- BPIFF antibody
- CETP antibody
- CETP_HUMAN antibody
see all
Images
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Anti-CETP antibody (ab19012)
Predicted band size: 70 kDa
Western blot analysis of CETP expresion in rabbit liver tissue lysate. -
ICC/IF image of ab19012 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19012, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Cho KH et al. Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double-Blinded, and Placebo-Controlled Study. Oxid Med Cell Longev 2018:4809525 (2018). Read more (PubMed: 29854085) »
- Park KH et al. Slim Body Weight Is Highly Associated With Enhanced Lipoprotein Functionality, Higher HDL-C, and Large HDL Particle Size in Young Women. Front Endocrinol (Lausanne) 9:406 (2018). Read more (PubMed: 30072955) »