Anti-CETP antibody (ab19012)

Submit a review Q&A (4)References (5)

Overview

  • Product name
    Anti-CETP antibody
    See all CETP primary antibodies
  • Description
    Rabbit polyclonal to CETP
  • Host species
    Rabbit
  • Specificity
    This antibody recognises CETB (70 kDa).
  • Tested applications
    Suitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to Human CETP (N terminal). Immunogen is around amino acid 153.
    Database link: P11597

Properties

Applications

Our Abpromise guarantee covers the use of ab19012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
WB
  • Application notes
    ELISA: Use at an assay dependent dilution (PMID 19287187).
    WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 70 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.
    • Tissue specificity
      Expressed by the liver and secreted in plasma.
    • Involvement in disease
      Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
      Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.
    • Sequence similarities
      Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
    • Cellular localization
      Secreted > extracellular space.
    • Information by UniProt
    • Database links
      • Alternative names
        • BPIFF antibody
        • CETP antibody
        • CETP_HUMAN antibody
        • Cholesteryl ester transfer protein antibody
        • Cholesteryl ester transfer protein plasma antibody
        • HDLCQ10 antibody
        • Lipid transfer protein I antibody
        see all

      Images

      • Western blot - Anti-CETP antibody (ab19012)
        Western blot - Anti-CETP antibody (ab19012)
        Anti-CETP antibody (ab19012)
        Predicted band size: 70 kDa



        Western blot analysis of CETP expresion in rabbit liver tissue lysate.
      • Immunocytochemistry/ Immunofluorescence - Anti-CETP antibody (ab19012)
        Immunocytochemistry/ Immunofluorescence - Anti-CETP antibody (ab19012)
        ICC/IF image of ab19012 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19012, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      References

      This product has been referenced in:
      • Cho KH  et al. Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double-Blinded, and Placebo-Controlled Study. Oxid Med Cell Longev 2018:4809525 (2018). Read more (PubMed: 29854085) »
      • Park KH  et al. Slim Body Weight Is Highly Associated With Enhanced Lipoprotein Functionality, Higher HDL-C, and Large HDL Particle Size in Young Women. Front Endocrinol (Lausanne) 9:406 (2018). Read more (PubMed: 30072955) »
      See all 5 Publications for this product

      Publishing research using ab19012? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Question

      Attached please find the image of the WB film. (Or do you mean you would like to see the image of the stained gel?) Thanks for your help

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      Answer

      Thank you very much for forwarding your Western blot image. The first three samples make a lot of sense to me. The protein is secreted, so I would expect to see much more protein present in serum than in cell or tissue lysates. As I said before, it may be that the protein in your samples is more highly glycosylated than the samples tested (rabbit liver is shown on the datasheet). Also, apparent molecular weight can vary from lab to lab due to differences in reagents. This wouldn't account for such a large difference by itself, but in this case, it may be contributing. In addition, the band above ~50 kDa could be the unglycosylated protein. Again, for the purified protein, I would load much less, maybe try 10 and 100 ng instead of microgram amounts. You may wish to play around with the incubation and blocking conditions as well, such as diluting out the antibody further but also removing the blocking agent from the primary antibody solution. Please let me know if my suggestions helps, and do not hesitate to contact us if you need any more advice or information.

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      Question

      It is curious that ab19012 reacts with rat CETP as neither rats nor mice have plasma CETP activity or a functional CETP gene (e.g. Comp. Biochem. Physiol. 135 (2003) 71-81).

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      Answer

      Thank you for your enquiry regarding ab19012 and your interest in our products. There are controversial data regarding whether mouse and rat have the CETP activity or proteins. We have used the mouse intestine and rat kidney, both of which show the specific 70 kDa bands. I hope this information will be useful for you.

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      Question

      When this antibody was tested in Western blotting, how was the sample prepared (the positive control lysate), how much protein was loaded onto the gel, and was a nitrocellulose or pvdf membrane used?

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      Answer

      Thank you for your enquiry. Ab19012 was indeed tested using rabbit liver tissue lysate, and a PVDF membrane was used. The lysate was prepared by directly sonicating the tissue pieces in SDS-PAGE sample buffer. 12% gel was used and 30 ug protein was loaded. Please contact us again if you have any additional questions.

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      Question

      Does the CETP antibody (ab 19012) work in hamster liver sample for western blot detection? If yes, which secondary antibody is the best choice for western blot detection? May I have detail information about that secondary antibody?

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      Answer

      Thank you for your email. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with hamster has not yet been tested for use with ab19012. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 points will be offered for an image). At the bottom of the online datasheet for ab19012 is a list of compatible secondary antibodies. A suitable secondary antibody to use with this primary for Western blotting is ab6721 - Goat polyclonal to Rabbit IgG H&L (HRP). Please let me know if you require any further assistance.

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