Anti-CETP antibody [ATM192] (ab2726)

Reviews (3)Q&A (3)References (4)

Key features and details

  • Mouse monoclonal [ATM192] to CETP
  • Suitable for: ELISA, IP, WB, ICC/IF
  • Reacts with: Rabbit, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CETP antibody [ATM192]
    See all CETP primary antibodies

  • Description

    Mouse monoclonal [ATM192] to CETP

  • Host species

    Mouse

  • Specificity

    Detects cholesteryl ester transfer protein (CETP) from human and rabbit tissues.

  • Tested applications

    Suitable for: ELISA, IP, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Rabbit, Human
    Predicted to work with: Cynomolgus monkey

  • Immunogen

    Synthetic peptide corresponding to Human CETP aa 149-159.
    Sequence:

    CDSGRVRTDAPD

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB,IP: human serum WB: Recombinant Human CETP protein (ab114408), human liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab2726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration. Use protein G beads.
WB 1/400. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/20.

Target

  • Function

    Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.

  • Tissue specificity

    Expressed by the liver and secreted in plasma.

  • Involvement in disease

    Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
    Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.

  • Sequence similarities

    Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • BPIFF antibody
      • CETP antibody
      • CETP_HUMAN antibody
      • Cholesteryl ester transfer protein antibody
      • Cholesteryl ester transfer protein plasma antibody
      • HDLCQ10 antibody
      • Lipid transfer protein I antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CETP antibody [ATM192] (ab2726)
      Immunocytochemistry/ Immunofluorescence - Anti-CETP antibody [ATM192] (ab2726)

      Immunocytochemistry/Immunofluorescence analysis of CETP in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2726 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. CETP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

    • Western blot - Anti-CETP antibody [ATM192] (ab2726)
      Western blot - Anti-CETP antibody [ATM192] (ab2726)This image is courtesy of an anonymous Abreview
      Anti-CETP antibody [ATM192] (ab2726) at 1/500 dilution + Whole cell lysate from rabbit fibroblasts at 40 µg

      Secondary
      HRP-conjugated rabbit anti-mouse at 1/2000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 70 kDa


      Exposure time: 2 minutes

      See Abreview

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab2726? Please let us know so that we can cite the reference in this datasheet.

    ab2726 has been referenced in 4 publications.

    • Cho KH  et al. Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double-Blinded, and Placebo-Controlled Study. Oxid Med Cell Longev 2018:4809525 (2018). PubMed: 29854085
    • Park KH  et al. Slim Body Weight Is Highly Associated With Enhanced Lipoprotein Functionality, Higher HDL-C, and Large HDL Particle Size in Young Women. Front Endocrinol (Lausanne) 9:406 (2018). PubMed: 30072955
    • Niculescu LS  et al. Apolipoprotein A-I stimulates cholesteryl ester transfer protein and apolipoprotein E secretion from lipid-loaded macrophages; the role of NF-?B and PKA signaling pathways. Biochem Biophys Res Commun 415:497-502 (2011). WB ; Human . PubMed: 22056562
    • Thomas AP  et al. Mouse monoclonal antipeptide antibodies specific for cholesteryl ester transfer protein (CETP). Hybridoma 15:359-64 (1996). PubMed: 8913785

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-CETP antibody [ATM192]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human THP-1 macrophages)
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Loading amount
    70 µg
    Treatment
    100 µg/ml oxidized LDL (oxLDL) +/- 10 µg/ml apoA-I
    Specification
    Human THP-1 macrophages
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Anca Sima

    Verified customer

    Submitted Jun 04 2015

    Western blot abreview for Anti-CETP [ATM192] antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% tris-glycine)
    Sample
    Cynomolgus Monkey Serum (serum, PBS diluted)
    Specification
    serum, PBS diluted
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 30 2014

    Western blot abreview for Anti-CETP antibody [ATM192]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Cell lysate - whole cell (Fibroblasts)
    Loading amount
    40 µg
    Specification
    Fibroblasts
    Treatment
    virus
    Gel Running Conditions
    Reduced Denaturing (gel 10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 02 2008

    Question

    One customer have bought one vial ab2726(CETP antibody ), the lot No is 72205. She used it to test CETP expressed in COS7 cells( transfected with full lenght human or animal CETP gene) at 1:400(recommendated by datasheet). But the result was not good: no bands with short exposure time, then elonged to 15mins, several bands appeared with high backgroud. the detect system is proved to work with other primary antibody. Then, she used another CETP polyclonal antibody prepared by themself to test as contrast. the result is good, clear target bands can be showed( see attchment for result picture) . Can you give me your suggestions to solve this problem asap?

    Read More
    Answer

    I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the secondary (anti mouse antibody) with other antibodies and on its own? Thank you for your patience, I look forward to hearing from you,

    Read More

    Question

    One customer have bought one vial ab2726(CETP antibody ), the lot No is 72205. She used it to test CETP expressed in COS7 cells( transfected with full lenght human or animal CETP gene) at 1:400(recommendated by datasheet). But the result was not good: no bands with short exposure time, then elonged to 15mins, several bands appeared with high backgroud. the detect system is proved to work with other primary antibody. Then, she used another CETP polyclonal antibody prepared by themself to test as contrast. the result is good, clear target bands can be showed( see attchment for result picture) . Can you give me your suggestions to solve this problem asap?

    Read More
    Answer

    I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the secondary (anti mouse antibody) with other antibodies and on its own? Thank you for your patience, I look forward to hearing from you,

    Read More

    Question

    Customer would like to know the following regarding ab2726: Is it a kappa or lambda? What is the affinity? Was it used in direct or coating ELISA?

    Read More
    Answer

    The antibody is IgG1. We have not further characterized the antibody in terms of Kappa and Lamda. Here is a refernce for ELISA: A.P. Thomas Mouse Monoclonal Antipeptide Antibodies Specific for Cholesteryl Ester Transfer Protein (CETP). Hybridoma 15(5): 359-364, 1996.

    Read More

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