Overview

  • Product name
    Anti-CETP antibody [ATM192]
    See all CETP primary antibodies
  • Description
    Mouse monoclonal [ATM192] to CETP
  • Host species
    Mouse
  • Specificity
    Detects cholesteryl ester transfer protein (CETP) from human and rabbit tissues.
  • Tested applications
    Suitable for: ELISA, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human CETP aa 149-159.
    Sequence:

    CDSGRVRTDAPD

  • Positive control
    • WB,IP: human serum WB: human liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab2726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration. Use protein G beads.
WB 1/400. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/20.

Target

  • Function
    Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.
  • Tissue specificity
    Expressed by the liver and secreted in plasma.
  • Involvement in disease
    Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
    Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.
  • Sequence similarities
    Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • BPIFF antibody
    • CETP antibody
    • CETP_HUMAN antibody
    • Cholesteryl ester transfer protein antibody
    • Cholesteryl ester transfer protein plasma antibody
    • HDLCQ10 antibody
    • Lipid transfer protein I antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of CETP in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2726 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. CETP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Anti-CETP antibody [ATM192] (ab2726) at 1/500 dilution + Whole cell lysate from rabbit fibroblasts at 40 µg

    Secondary
    HRP-conjugated rabbit anti-mouse at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa


    Exposure time: 2 minutes

    See Abreview

References

This product has been referenced in:
  • Cho KH  et al. Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double-Blinded, and Placebo-Controlled Study. Oxid Med Cell Longev 2018:4809525 (2018). Read more (PubMed: 29854085) »
  • Niculescu LS  et al. Apolipoprotein A-I stimulates cholesteryl ester transfer protein and apolipoprotein E secretion from lipid-loaded macrophages; the role of NF-?B and PKA signaling pathways. Biochem Biophys Res Commun 415:497-502 (2011). WB ; Human . Read more (PubMed: 22056562) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Human THP-1 macrophages)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
70 µg
Treatment
100 µg/ml oxidized LDL (oxLDL) +/- 10 µg/ml apoA-I
Specification
Human THP-1 macrophages
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Anca Sima

Verified customer

Submitted Jun 04 2015

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycine)
Sample
Cynomolgus Monkey Serum (serum, PBS diluted)
Specification
serum, PBS diluted
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 30 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Cell lysate - whole cell (Fibroblasts)
Loading amount
40 µg
Specification
Fibroblasts
Treatment
virus
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 02 2008

I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the...

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I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the...

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The antibody is IgG1. We have not further characterized the antibody in terms of Kappa and Lamda. Here is a refernce for ELISA: A.P. Thomas Mouse Monoclonal Antipeptide Antibodies Specific for Cholesteryl Ester Transfer Protein (CETP). Hybridoma...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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