Anti-CETP antibody [ATM192] (ab2726)
Key features and details
- Mouse monoclonal [ATM192] to CETP
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CETP antibody [ATM192]
See all CETP primary antibodies -
Description
Mouse monoclonal [ATM192] to CETP -
Host species
Mouse -
Specificity
Detects cholesteryl ester transfer protein (CETP) from human and rabbit tissues. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Human CETP aa 149-159.
Sequence:CDSGRVRTDAPD
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
ATM192 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/20.
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WB | (3) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 55 kDa.
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Notes |
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ICC/IF
1/20. |
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 55 kDa. |
Target
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Function
Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels. -
Sequence similarities
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 1071 Human
- Omim: 118470 Human
- SwissProt: P11597 Human
- Unigene: 89538 Human
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Alternative names
- BPIFF antibody
- CETP antibody
- CETP_HUMAN antibody
see all
Images
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All lanes : Anti-CETP antibody [ATM192] (ab2726) at 2 µg/ml
Lane 1 : HT-29 whole cell extracts
Lane 2 : HCT 116 whole cell extracts
Secondary
All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjµgate at 0.4 µg/ml
Predicted band size: 55 kDa -
Immunocytochemistry/Immunofluorescence analysis of CETP in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2726 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. CETP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab2726 has been referenced in 4 publications.
- Cho KH et al. Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double-Blinded, and Placebo-Controlled Study. Oxid Med Cell Longev 2018:4809525 (2018). PubMed: 29854085
- Park KH et al. Slim Body Weight Is Highly Associated With Enhanced Lipoprotein Functionality, Higher HDL-C, and Large HDL Particle Size in Young Women. Front Endocrinol (Lausanne) 9:406 (2018). PubMed: 30072955
- Niculescu LS et al. Apolipoprotein A-I stimulates cholesteryl ester transfer protein and apolipoprotein E secretion from lipid-loaded macrophages; the role of NF-?B and PKA signaling pathways. Biochem Biophys Res Commun 415:497-502 (2011). WB ; Human . PubMed: 22056562
- Thomas AP et al. Mouse monoclonal antipeptide antibodies specific for cholesteryl ester transfer protein (CETP). Hybridoma 15:359-64 (1996). PubMed: 8913785