Overview

  • Product name

    Anti-CETP antibody [ATM192]
    See all CETP primary antibodies
  • Description

    Mouse monoclonal [ATM192] to CETP
  • Host species

    Mouse
  • Specificity

    Detects cholesteryl ester transfer protein (CETP) from human and rabbit tissues.
  • Tested applications

    Suitable for: ELISA, IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rabbit, Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human CETP aa 149-159.
    Sequence:

    CDSGRVRTDAPD

  • Positive control

    • WB,IP: human serum WB: Recombinant Human CETP protein (ab114408), human liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab2726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration. Use protein G beads.
WB 1/400. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/20.

Target

  • Function

    Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Defects in CETP are a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL).
    Defects in CETP are the cause of cholesteryl ester transfer protein deficiency (CETP deficiency) [MIM:607322]. This is an autosomal dominant condition associated with increased HDL cholesterol levels.
  • Sequence similarities

    Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • BPIFF antibody
    • CETP antibody
    • CETP_HUMAN antibody
    • Cholesteryl ester transfer protein antibody
    • Cholesteryl ester transfer protein plasma antibody
    • HDLCQ10 antibody
    • Lipid transfer protein I antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of CETP in HepG2 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2726 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. CETP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Anti-CETP antibody [ATM192] (ab2726) at 1/500 dilution + Whole cell lysate from rabbit fibroblasts at 40 µg

    Secondary
    HRP-conjugated rabbit anti-mouse at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa


    Exposure time: 2 minutes

    See Abreview

References

This product has been referenced in:

  • Cho KH  et al. Consumption of Cuban Policosanol Improves Blood Pressure and Lipid Profile via Enhancement of HDL Functionality in Healthy Women Subjects: Randomized, Double-Blinded, and Placebo-Controlled Study. Oxid Med Cell Longev 2018:4809525 (2018). Read more (PubMed: 29854085) »
  • Park KH  et al. Slim Body Weight Is Highly Associated With Enhanced Lipoprotein Functionality, Higher HDL-C, and Large HDL Particle Size in Young Women. Front Endocrinol (Lausanne) 9:406 (2018). Read more (PubMed: 30072955) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Q&A

Answer

I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the secondary (anti mouse antibody) with other antibodies and on its own? Thank you for your patience, I look forward to hearing from you,

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Answer

I'm sorry to hear your customer is having a problem with ab2726. Could you please tell me what blocking buffer was used (time and temperature too) and what primary incubation buffer was used too (with time and temperature). Has the customer tested the secondary (anti mouse antibody) with other antibodies and on its own? Thank you for your patience, I look forward to hearing from you,

Read More

Answer

The antibody is IgG1. We have not further characterized the antibody in terms of Kappa and Lamda. Here is a refernce for ELISA: A.P. Thomas Mouse Monoclonal Antipeptide Antibodies Specific for Cholesteryl Ester Transfer Protein (CETP). Hybridoma 15(5): 359-364, 1996.

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