CF405M Anti-CD105 antibody [2H6F11], prediluted (ab123634)
![Flow Cytometry - CF405M Anti-CD105 antibody [2H6F11], prediluted (ab123634)](https://www.abcam.com/ps/products/123/ab123634/Images/CD105-Primary-antibodies-ab123634-1.jpg "Flow Cytometry - CF405M Anti-CD105 antibody [2H6F11], prediluted (ab123634)")
Key features and details
- CF405M Mouse monoclonal [2H6F11] to CD105, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: CF405M. Ex: 408nm, Em: 452nm
- Isotype: IgG1
Overview
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Product name
CF405M Anti-CD105 antibody [2H6F11], prediluted
See all CD105 primary antibodies -
Description
CF405M Mouse monoclonal [2H6F11] to CD105, prediluted -
Host species
Mouse -
Conjugation
CF405M. Ex: 408nm, Em: 452nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Human Endoglin fusion protein.
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Positive control
- Nalm-6 cell line
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General notes
CD105 is a component of the TGF-B receptor system and can bind TGF-B1 and TGF-B3 with high affinity but does not bind TGF-B2. CD105 is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta, Pre-B cells in fetal marrow, erythroid precursors in fetal and adult bone marrow and is weakly expressed by stromal fibroblasts. U937 cells and monocytes and tissue macrophages also expresses weakly CD105. Expression of CD105 is increased on activated endothelium in tissues undergoing angiogenesis, such as in tumours, or in cases of wound healing or dermal inflammation.
CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
CD105 is a component of the TGF-B receptor system and can bind TGF-B1 and TGF-B3 with high affinity but does not bind TGF-B2. CD105 is expressed by vascular endothelial cells and syncytiotrophoblasts of placenta, Pre-B cells in fetal marrow, erythroid precursors in fetal and adult bone marrow and is weakly expressed by stromal fibroblasts. U937 cells and monocytes and tissue macrophages also expresses weakly CD105. Expression of CD105 is increased on activated endothelium in tissues undergoing angiogenesis, such as in tumours, or in cases of wound healing or dermal inflammation. -
Clonality
Monoclonal -
Clone number
2H6F11 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 5µl for 106 cells.
ab126026 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 5µl for 106 cells. ab126026 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
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Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
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Flow Cytometry - CF405M Anti-CD105 antibody [2H6F11], prediluted (ab123634)Staining of Nalm-6 cell line with ab123634 at 5 µl/106 cells and isotype control IgG1 CFBlue. Total cells were used for analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab123634 has not yet been referenced specifically in any publications.