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  1. Link

    cf405m-cd15-antibody-mcs-1-prediluted-ab123633.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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CF405M Anti-CD15 antibody [MCS-1], prediluted (ab123633)

  • Datasheet
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Flow Cytometry - CF405M Anti-CD15 antibody [MCS-1], prediluted (ab123633)
  • Flow Cytometry - CF405M Anti-CD15 antibody [MCS-1], prediluted (ab123633)

Key features and details

  • CF405M Mouse monoclonal [MCS-1] to CD15, prediluted
  • Suitable for: Flow Cyt
  • Reacts with: Species independent
  • Conjugation: CF405M. Ex: 408nm, Em: 452nm
  • Isotype: IgG3

Overview

  • Product name

    CF405M Anti-CD15 antibody [MCS-1], prediluted
    See all CD15 primary antibodies
  • Description

    CF405M Mouse monoclonal [MCS-1] to CD15, prediluted
  • Host species

    Mouse
  • Conjugation

    CF405M. Ex: 408nm, Em: 452nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    White blood cells

  • Positive control

    • Normal Human Peripheral Blood
  • General notes

    CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS

    pH +/-0.2
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    MCS-1
  • Isotype

    IgG3
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Immunology
    • Cell Type Markers
    • CD
    • Adhesion
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Cancer
    • Tumor immunology
    • CD markers
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers

Associated products

  • Alternative Versions

    • APC Anti-CD15 antibody [MCS-1] (ab91151)

Applications

Our Abpromise guarantee covers the use of ab123633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells.

ab126029 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.
  • Cellular localization

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.
  • Alternative names

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

Images

  • Flow Cytometry - CF405M Anti-CD15 antibody [MCS-1], prediluted (ab123633)
    Flow Cytometry - CF405M Anti-CD15 antibody [MCS-1], prediluted (ab123633)
    Flow cytometry analysis of CD15 expression in Normal Peripheral Blood from a Human Donor using ab123633 at 5 µl/106 cells.
  • Flow Cytometry - CF405M Anti-CD15 antibody [MCS-1], prediluted (ab123633)
    Flow Cytometry - CF405M Anti-CD15 antibody [MCS-1], prediluted (ab123633)
    Flow cytometry analysis of CD15 expression in Normal Peripheral Blood from a Human Donor using ab123633 at 5 µl/106 cells.

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab123633? Please let us know so that we can cite the reference in this datasheet.

    ab123633 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Flow Cytometry abreview for Anti-CD15 antibody [MCS-1] (CF405M), prediluted

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    None
    Permeabilization
    No
    Sample
    Sheep Cell (Peripheral blood)
    Specification
    Peripheral blood
    Gating Strategy
    Granulocytes
    Preparation
    Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
    Sample buffer: None
    Read More

    Ms. Ina Laura Pieper

    Verified customer

    Submitted Sep 16 2013

    Flow Cytometry abreview for Anti-CD15 antibody [MCS-1] (CF405M), prediluted

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    None
    Permeabilization
    No
    Sample
    Cow Cell (Peripheral blood)
    Specification
    Peripheral blood
    Gating Strategy
    Granulocytes
    Preparation
    Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
    Sample buffer: None
    Read More

    Ms. Ina Laura Pieper

    Verified customer

    Submitted Sep 16 2013

    Flow Cytometry abreview for Anti-CD15 antibody [MCS-1] (CF405M), prediluted

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    None
    Permeabilization
    No
    Sample
    Human Cell (Peripheral blood)
    Specification
    Peripheral blood
    Gating Strategy
    Granulocytes
    Preparation
    Cell harvesting/tissue preparation method: Sodium citrate anticoagulated fresh blood
    Sample buffer: None
    Read More

    Ms. Ina Laura Pieper

    Verified customer

    Submitted Sep 11 2013

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