CF405M Anti-CD3 epsilon antibody [145-2C11] (ab236573)

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Flow Cytometry - CF405M Anti-CD3 epsilon antibody [145-2C11] (ab236573)

    Key features and details

    • CF405M Armenian hamster monoclonal [145-2C11] to CD3 epsilon
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: CF405M. Ex: 408nm, Em: 452nm
    • Isotype: IgG

    Overview

    • Product name

      CF405M Anti-CD3 epsilon antibody [145-2C11]
      See all CD3 epsilon primary antibodies

    • Description

      CF405M Armenian hamster monoclonal [145-2C11] to CD3 epsilon

    • Host species

      Armenian hamster

    • Conjugation

      CF405M. Ex: 408nm, Em: 452nm

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      Tissue, cells or virus corresponding to CD3 epsilon. (H-2Kb specific cytotoxic T lymphocyte clone BM10-37).

    • Positive control

      • Flow: Mouse splenocytes.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab236573 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 1µg for 106 cells.

    Target

    • Function

      The CD3 complex mediates signal transduction.

    • Sequence similarities

      Contains 1 Ig-like (immunoglobulin-like) domain.
      Contains 1 ITAM domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD3 epsilon antibody
        • CD3e antibody
        • CD3e antigen antibody
        • CD3e antigen epsilon polypeptide (TiT3 complex) antibody
        • CD3E antigen epsilon polypeptide antibody
        • CD3E antigen, epsilon subunit antibody
        • CD3e molecule epsilon antibody
        • CD3e molecule, epsilon (CD3 TCR complex) antibody
        • CD3e molecule, epsilon (CD3-TCR complex) antibody
        • CD3E_HUMAN antibody
        • IMD18 antibody
        • T cell antigen receptor complex epsilon subunit of T3 antibody
        • T cell surface antigen T3/Leu 4 epsilon chain antibody
        • T cell surface glycoprotein CD3 epsilon chain antibody
        • T-cell surface antigen T3/Leu-4 epsilon chain antibody
        • T-cell surface glycoprotein CD3 epsilon chain antibody
        • T3E antibody
        • TCRE antibody
        see all

      Images

      • Flow Cytometry - CF405M Anti-CD3 epsilon antibody [145-2C11] (ab236573)
        Flow Cytometry - CF405M Anti-CD3 epsilon antibody [145-2C11] (ab236573)

        Flow cytometric analysis of mouse splenocytes stained with ab236573 (filled pink histogram) or Armenian hamster IgG CF-Blue isotype control (brown histogram).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab236573? Please let us know so that we can cite the reference in this datasheet.

      ab236573 has not yet been referenced specifically in any publications.

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