CF405M Anti-CD4 antibody [GK1.5] (ab237029)
![Flow Cytometry - CF405M Anti-CD4 antibody [GK1.5] (ab237029)](https://www.abcam.com/ps/products/237/ab237029/Images/ab237029-314972-anti-cd4-antibody-gk15-cf405m-flow-cytometry.jpg "Flow Cytometry - CF405M Anti-CD4 antibody [GK1.5] (ab237029)")
Key features and details
- CF405M Rat monoclonal [GK1.5] to CD4
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: CF405M. Ex: 408nm, Em: 452nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
CF405M Anti-CD4 antibody [GK1.5]
See all CD4 primary antibodies -
Description
CF405M Rat monoclonal [GK1.5] to CD4 -
Host species
Rat -
Conjugation
CF405M. Ex: 408nm, Em: 452nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD4. (Mouse CTL clone V4).
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Positive control
- Flow Cyt: Mouse splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from an in vitro cell culture of a cell hybridoma. -
Clonality
Monoclonal -
Clone number
GK1.5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
|
| Notes |
|---|
|
Flow Cyt
Use 1µg for 106 cells. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 12504 Mouse
- SwissProt: P06332 Mouse
- Unigene: 2209 Mouse
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Flow Cytometry - CF405M Anti-CD4 antibody [GK1.5] (ab237029)
Flow cytometric analysis of mouse splenocytes stained for CD4 with ab237029 (filled histogram) or rat IgG2b, κ CF405M isotype control (blue histogram).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab237029 has not yet been referenced specifically in any publications.
