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  1. Link

    cf405m-cd4-antibody-gk15-ab237029.pdf

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Immunology Adaptive Immunity T Cells CD
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CF405M Anti-CD4 antibody [GK1.5] (ab237029)

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Flow Cytometry - CF405M Anti-CD4 antibody [GK1.5] (ab237029)

    Key features and details

    • CF405M Rat monoclonal [GK1.5] to CD4
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: CF405M. Ex: 408nm, Em: 452nm
    • Isotype: IgG2b

    Overview

    • Product name

      CF405M Anti-CD4 antibody [GK1.5]
      See all CD4 primary antibodies
    • Description

      CF405M Rat monoclonal [GK1.5] to CD4
    • Host species

      Rat
    • Conjugation

      CF405M. Ex: 408nm, Em: 452nm
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Mouse
    • Immunogen

      Tissue, cells or virus corresponding to Mouse CD4. (Mouse CTL clone V4).

    • Positive control

      • Flow Cyt: Mouse splenocytes.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      Purified from an in vitro cell culture of a cell hybridoma.
    • Clonality

      Monoclonal
    • Clone number

      GK1.5
    • Isotype

      IgG2b
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Immunology
      • Adaptive Immunity
      • Regulatory T Cells
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • HIV-related

    Associated products

    • Alternative Versions

      • PerCP/Cy5.5® Anti-CD4 antibody [GK1.5] (ab210265)
      • FITC Anti-CD4 antibody [GK1.5] (ab218745)
      • violetFluor™ 450 Anti-CD4 antibody [GK1.5] (ab242009)
      • redFluor™ 710 Anti-CD4 antibody [GK1.5] (ab242010)
      • PE/Cy5® Anti-CD4 antibody [GK1.5] (ab25476)
      • APC Anti-CD4 antibody [GK1.5] (ab25497)

    Applications

    Our Abpromise guarantee covers the use of ab237029 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 1µg for 106 cells.

    Target

    • Function

      Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
    • Sequence similarities

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
    • Cellular localization

      Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Target information above from: UniProt accession P01730 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12504 Mouse
      • SwissProt: P06332 Mouse
      • Unigene: 2209 Mouse
      • Alternative names

        • CD 4 antibody
        • CD4 (L3T4) antibody
        • CD4 antibody
        • CD4 antigen (p55) antibody
        • CD4 antigen antibody
        • CD4 molecule antibody
        • CD4 receptor antibody
        • CD4+ Lymphocyte deficiency, included antibody
        • CD4_HUMAN antibody
        • CD4mut antibody
        • L3T4 antibody
        • Leu3 antibody
        • Ly-4 antibody
        • Lymphocyte antigen CD4 antibody
        • MGC165891 antibody
        • OTTHUMP00000238897 antibody
        • p55 antibody
        • T cell antigen T4 antibody
        • T cell antigen T4/LEU3 antibody
        • T cell differentiation antigen L3T4 antibody
        • T cell OKT4 deficiency, included antibody
        • T cell surface antigen T4/Leu 3 antibody
        • T cell surface antigen T4/Leu3 antibody
        • T cell surface glycoprotein CD4 antibody
        • T-cell surface antigen T4/Leu-3 antibody
        • T-cell surface glycoprotein CD4 antibody
        • W3/25 antibody
        • W3/25 antigen antibody
        see all

      Images

      • Flow Cytometry - CF405M Anti-CD4 antibody [GK1.5] (ab237029)
        Flow Cytometry - CF405M Anti-CD4 antibody [GK1.5] (ab237029)

        Flow cytometric analysis of mouse splenocytes stained for CD4 with ab237029 (filled histogram) or rat IgG2b, κ CF405M isotype control (blue histogram).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab237029? Please let us know so that we can cite the reference in this datasheet.

      ab237029 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab237029.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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