CF405M Anti-CD43 antibody [TP1/36] (ab119482)
![Flow Cytometry - CF405M Anti-CD43 antibody [TP1/36] (ab119482)](https://www.abcam.com/ps/products/119/ab119482/Images/CD43-Primary-antibodies-ab119482-3.jpg "Flow Cytometry - CF405M Anti-CD43 antibody [TP1/36] (ab119482)")
Key features and details
- CF405M Mouse monoclonal [TP1/36] to CD43
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: CF405M. Ex: 408nm, Em: 452nm
- Isotype: IgG1
Overview
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Product name
CF405M Anti-CD43 antibody [TP1/36]
See all CD43 primary antibodies -
Description
CF405M Mouse monoclonal [TP1/36] to CD43 -
Host species
Mouse -
Conjugation
CF405M. Ex: 408nm, Em: 452nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
T blasts activated with PMA and ionomycin (Human)
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Positive control
- Normal Human peripheral blood cells.
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General notes
CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Buffer containing antibody stabilizer solution. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
TP1/36 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab119482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab126026 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
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Function
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
Tissue specificity
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
Post-translational
modificationsGlycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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Alternative names
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
Images
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Flow Cytometry - CF405M Anti-CD43 antibody [TP1/36] (ab119482)ab119482, at 5 µl/106 cells, staining CD43 in normal Human peripheral blood cells by Flow Cytometry. Cells in the total leukocytes were used for the analysis.
Datasheets and documents
References (0)
ab119482 has not yet been referenced specifically in any publications.
