Key features and details
- CF405M Mouse monoclonal [VJ1/12.2] to CD59
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: CF405M. Ex: 408nm, Em: 452nm
- Isotype: IgG2a
Product nameCF405M Anti-CD59 antibody [VJ1/12.2]
See all CD59 primary antibodies
DescriptionCF405M Mouse monoclonal [VJ1/12.2] to CD59
ConjugationCF405M. Ex: 408nm, Em: 452nm
Specificityab117734 is recommended for use in flow cytometry for identification of all types of leukocytes including platelets.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
HUVEC cellsTNF activated
- Normal Human peripheral blood cells
ab117734 reacts with a 19kDa glycosylphosphatidylinositol (GPI) linked glycoprotein expressed on haematopoietic and non-haematopoietic cells. CD59 is expressed, like CD55, in erythrocytes. Recognizes a carbohydrate antigen present on a subset of peripheral blood mononuclear cells involved in the natural killer activity, which is not MHC restricted. The antigen is not present on B cells, monocytes, red blood cells or platelets but is expressed on cells in neuroectodermal tissue. It reacts with 20% of normal Human peripheral blood mononuclear cells.
CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesab117734 reacts with a 19kDa glycosylphosphatidylinositol (GPI) linked glycoprotein expressed on haematopoietic and non-haematopoietic cells. CD59 is expressed, like CD55, in erythrocytes. Recognizes a carbohydrate antigen present on a subset of peripheral blood mononuclear cells involved in the natural killer activity, which is not MHC restricted. The antigen is not present on B cells, monocytes, red blood cells or platelets but is expressed on cells in neuroectodermal tissue. It reacts with 20% of normal Human peripheral blood mononuclear cells.
Our Abpromise guarantee covers the use of ab117734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 5µl for 106 cells.
ab126036 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionPotent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
Involvement in diseaseDefects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
Sequence similaritiesContains 1 UPAR/Ly6 domain.
modificationsN- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
Cellular localizationCell membrane. Secreted. Soluble form found in a number of tissues.
- Information by UniProt
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ab117734 has been referenced in 1 publication.
- Thielen AJF et al. Complement Deposition and IgG Binding on Stored Red Blood Cells Are Independent of Storage Time. Transfus Med Hemother 45:378-384 (2018). PubMed: 30574054