CF405M Anti-DPP4 antibody [TP1/19] (ab119480)
![Flow Cytometry - CF405M Anti-DPP4 antibody [TP1/19] (ab119480)](https://www.abcam.com/ps/products/119/ab119480/Images/ab119480-132795-anti-cd26-antibody-tp1-19-cf405m-flow-cytometry.jpg "Flow Cytometry - CF405M Anti-DPP4 antibody [TP1/19] (ab119480)")
Key features and details
- CF405M Mouse monoclonal [TP1/19] to DPP4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: CF405M. Ex: 408nm, Em: 452nm
- Isotype: IgG2b
Overview
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Product name
CF405M Anti-DPP4 antibody [TP1/19]
See all DPP4 primary antibodies -
Description
CF405M Mouse monoclonal [TP1/19] to DPP4 -
Host species
Mouse -
Conjugation
CF405M. Ex: 408nm, Em: 452nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human DPP4.
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Positive control
- Normal Human peripheral blood cells.
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General notes
CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Buffer containing antibody stabilizer solution. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
TP1/19 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab119480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 5µl for 106 cells. ab126033 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. -
Tissue specificity
Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. -
Sequence similarities
Belongs to the peptidase S9B family. DPPIV subfamily. -
Domain
The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity. -
Post-translational
modificationsThe soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
N- and O-Glycosylated.
Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. -
Cellular localization
Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid. - Information by UniProt
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Database links
- Entrez Gene: 1803 Human
- Omim: 102720 Human
- SwissProt: P27487 Human
- Unigene: 368912 Human
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Alternative names
- CD26 antigen antibody
- ADA-binding protein antibody
- ADABP antibody
see all
Images
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Flow Cytometry - CF405M Anti-DPP4 antibody [TP1/19] (ab119480)ab119480, at 5 µl/106 cells, staining CD26 in normal Human peripheral blood cells by Flow Cytometry. Cells in the leukocyte gate were used for the analysis.
Datasheets and documents
References (0)
ab119480 has not yet been referenced specifically in any publications.
